隔离,表征和应用一个针对Vibrio splendidus生物膜的Lytic菌体vB_VspM_VS1
Xuemei Duan1,2, Liming Jiang1,2,3, Ming Guo1,2
1State Key Laboratory for Quality and Safety of Agroproducts, Ningbo University, Ningbo, China.
PloS one
|September 1, 2023
概括
一种新的毒性菌体vB_VspM_VS1被分离出来,用于对抗海洋病原体Vibrio splendidus. 这种菌体有效抑制细菌生长和生物膜,为水产养殖提供潜在的生物控制剂.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 水产养殖科学 水产养殖科学
背景情况:
- 斑杆菌 (Vibrio splendidus) 是一种重要的海洋病原体,影响了像Apostichopus japonicus这样的水产养殖物种.
- 目前对V. splendidus感染的治疗方法有限,因此需要采用替代的控制策略.
研究的目的:
- 为了隔离和鉴定向Vibrio splendidus的毒性细菌菌体.
- 为了阐明隔离的菌体的基因组特征和光学活性.
- 评估菌体对控制V. splendidus生长和生物膜的疗效.
主要方法:
- 从水产养殖池中分离了菌体vB_VspM_VS1.
- 菌体的特征包括吸附率,潜伏期和爆发大小.
- 全基因组测序和菌体的生物信息分析.
- 细菌生长抑制和生物膜破坏试验.
主要成果:
- 体vB_VspM_VS1,属于Straboviridae家族,证明了快速吸附 (96%在15分钟内).
- 菌体拥有248,270bp的双链DNA基因组与389个假定蛋白质编码基因.
- 在体外,vB_VspM_VS1显著抑制了V. splendidus的生长,并破坏了生物膜.
结论:
- 菌体vB_VspM_VS1是水产养殖中Vibrio splendidus生物控制的有希望的候选物.
- 基因组表征提供了对菌体的解机制的洞察.
- 进一步的研究可以探索养水生生物中疾病预防的体内应用.
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