相关实验视频

Updated: Jul 17, 2025

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

角大小和deltaA表达预测了沿着血统进展的成年神经干细胞决策

Laure Mancini^1,2, Boris Guirao³, Sara Ortica¹

¹Institut Pasteur, Université Paris Cité, CNRS UMR3738, Zebrafish Neurogenetics Unit, Team supported by the Ligue Nationale Contre le Cancer, Paris 75015, France.

Science advances

|September 1, 2023

概括

神经干细胞 (NSC) 的维护依赖于分裂时间和模式. 成年斑马鱼大脑中的角大小和DeltaA表达NSC预测自我更新与神经发生,揭示了不同的NSC亚群.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
干细胞生物学干细胞生物学

背景情况:

成年大脑中神经干细胞 (NSC) 的维护对神经功能至关重要.
NSC行为由激活频率和分裂模式来调节,但基本机制尚未完全理解.

研究的目的:

研究预测成年斑马鱼大脑中神经干细胞激活频率和分裂模式的因素.
阐明顶峰表面积和DeltaA表达在调节NSC命运决策中的作用.

主要方法:

在成年斑马鱼telencephalon中进行NSC的长期静脉成像.
对Notch信号通路组件 (Notch,DeltaA) 的药理和遗传操纵.
对NSC顶部大小和DeltaA表达的分析,以预测血统进展.

主要成果:

圆顶表面积和DeltaA表达预测NSC激活频率和分裂模式.
德尔塔A阴性NSC形成了一个自我更新池,经历了不对称的分裂.
这些分裂产生了自我更新的DeltaA阴性子女和神经性DeltaA阳性子女.
圆顶大小预测激活频率,不对称的划分是独立于Notch信号的.

结论:

成人大脑NSC被分层组织成不同的子群体,具有不同的命运.
角大小和DeltaA表达是NSC血统在体内进展的关键预测因素.
NSC自我更新和神经发生是由部分独立于Notch信号的机制调节的.

更多相关视频

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed Assay

Published on: February 14, 2025

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Published on: September 14, 2015

相关概念视频

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...