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生物物理研究SARS-CoV-2尖端蛋白和神经皮林-1之间的相互作用
Decheng Hou1,2, Wenpeng Cao1, Seonghan Kim1
1Department of Bioengineering, Lehigh University, Bethlehem, Pennsylvania, USA.
Protein science : a publication of the Protein Society
|September 1, 2023
概括
神经蛋白-1 (NRP1) 作为SARS-CoV-2附着的替代受体,表现出比ACE2更强的结合. 中和抗体向尖端蛋白质的目标.
科学领域:
- 病毒学和生物物理学
- 分子相互作用和细胞生物学
背景情况:
- 神经素-1 (NRP1) 已被提议作为SARS-CoV-2的潜在受体.
- 目前尚不清楚NRP1和SARS-CoV-2组件之间的精确生物物理相互作用.
研究的目的:
- 为了研究NRP1和SARS-CoV-2尖峰 (S) 蛋白碎片之间的相互作用的生物物理特征.
- 将NRP1与SARS-CoV-2的结合与ACE2的结合进行比较.
主要方法:
- 原子力显微镜 (AFM) 用于测量结合频率 (BF) 和解结合力.
- 使用结构建模,AlphaFold2和分子动力学模拟来预测和分析结合模式.
- 确定解离率以评估结合亲和力.
主要成果:
- 与ACE2相比,NRP1与SARS-CoV-2 S蛋白碎片 (RBD和三元体) 的结合频率更高.
- 与NRP1-RBD和ACE2-S三元体相互作用相比,NRP1-S三元体相互作用显示出更大的解结力.
- NRP1对S三元体具有更强的结合亲和力,与NRP1-RBD相比,解离率明显较低.
结论:
- NRP1作为SARS-CoV-2的替代受体,促进病毒与人体细胞的附着.
- 针对SARS-CoV-2 RBD的中和抗体可以有效地减少对ACE2和NRP1的结合.
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