病毒囊对废水中的RNA和DNA病毒基因组的保护作用
Katherine R Harrison1, Delaney Snead1, Anna Kilts1
1Department of Civil & Environmental Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.
Environmental science & technology
|September 1, 2023
概括
病毒体保护废水中的遗传物质,延长其检测时间. 这一发现对于精确的基于废水的流行病学和公共卫生监测至关重要.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 分子诊断学 分子诊断学
背景情况:
- 废水病毒度为公众健康和流行病学提供信息.
- 基于PCR测量的准确解释需要了解核酸稳定性.
- 病毒囊保护和基因组衰变率是关键因素.
研究的目的:
- 为了研究模型病毒基因组在废水中的持久性.
- 量化病毒囊对核酸稳定性的保护作用.
- 为了比较病毒基因组和传染性病毒的衰变率.
主要方法:
- 基板批量实验使用25°C的废水流入.
- 测量细胞外 (+) sRNA 和 dsDNA amplicon 的降解速率.
- 对封闭和裸体病毒核酸 (MS2和T4菌体) 的T90 (90%降解时间) 的评估.
主要成果:
- 外病毒 (+) sRNA 和 dsDNA 迅速降解 (分别在 15-19 分钟和 1.6-1.9 小时内降解 90%).
- 封装显著增加了核酸稳定性:T90增加了MS2 (+) sRNA的424倍,T4 dsDNA的52倍.
- 病毒基因组衰变反映了感染能力的丧失;核酸信号在无活化后消失.
结论:
- 在废水中检测到的大多数病毒基因组拷贝很可能是封装的.
- 废水病毒测量在很大程度上独立于PCR amplicon大小.
- 非外病毒的基因组衰变率与无活化率相关,有助于解释废水监测数据.
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