聚醇和酸之间的协同作用对抗抗生素耐药的沙门氏菌 spp
Dino César Pereira Garcez1, Gabrielle Ribeiro1, Marcelo Kominkiewicz1
1University of West Santa Catarina (UNOESC), Xanxerê, State of Santa Catarina, 89600-000, Brazil.
Journal of applied microbiology
|September 1, 2023
概括
酸 (BA) 和多聚 (PPy) 显示出对多耐药沙门氏菌海德堡 (SH) 和沙门氏菌明尼苏达 (SM) 隔离物的抗菌活性. 这些化合物还显示出潜在的协同效应,影响生物膜生产和细菌代谢.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 黑德堡沙门氏菌 (SH) 和明尼苏达沙门氏菌 (SM) 是重要的食物传播病原体.
- 沙门氏菌的多药耐药性 (MDR) 构成了日益严重的公共卫生挑战.
- 生物膜的形成有助于细菌的持久性和耐药性.
研究的目的:
- 为了鉴定海德堡沙门氏菌 (SH) 和明尼苏达沙门氏菌 (SM) 隔离物的耐药性和持久性.
- 评估酸 (BA) 和多聚烯 (PPy) 对这些分离物的抗菌疗效.
- 调查BA和PPy的协同作用潜力.
主要方法:
- 抗生素和排泄表达分析20个来自肉垃圾的沙门氏菌分离物.
- 确定BA和PPy的最小抑制/杀菌度 (MIC/MBC),协同活性和杀时动力学.
- 评估生物膜的产生,相关基因的存在,以及与细菌点蛋白质的分子对接.
主要成果:
- 所有分离物体都表现出多药性耐药性 (MDR);BA和PPy显示出显著的MIC/MBC值.
- 化合物对SH分离物表现出协同活性,在4小时内将细菌数量减少了3个日志.
- 大多数分离物产生生物膜,并检测出与生物膜形成相关的基因;化合物与必需的细菌蛋白相互作用.
结论:
- 酸 (BA) 和多聚 (PPy) 显示出对抗多药耐药,产生生物膜的海德堡沙门氏菌 (SH) 和明尼苏达沙门氏菌 (SM) 的抗菌活性.
- 观察到BA和PPy之间的潜在协同效应.
- 这些化合物显示出对控制食品生产环境中的耐药沙门氏菌菌株的前景.
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