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相关概念视频

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

446
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
446
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

450
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
450
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

722
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
722
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.1K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

837
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
837

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相关实验视频

Updated: Jul 17, 2025

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
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拉曼扩散有序光谱学 拉曼扩散有序光谱学

Robert W Schmidt1,2, Giulia Giubertoni2, Federico Caporaletti2,3

  • 1Vrije Universiteit Amsterdam, De Boelelaan 1105, 1081HV Amsterdam, The Netherlands.

The journal of physical chemistry. A
|September 1, 2023
PubMed
概括
此摘要是机器生成的。

拉曼扩散顺序光谱法 (Raman-DOSY) 将拉曼光谱法与扩散测量相结合,以确定分子大小. 这种新的技术可以解决不同大小的分子的重叠信号,为化学分析提供了一种通用的新工具.

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科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 物理化学 物理化学

背景情况:

  • 斯托克斯-爱因斯坦关系对于通过扩散系数来确定分子大小至关重要.
  • 拉曼光谱提供结构信息,但通常缺乏尺寸灵敏度.
  • 现有的基于扩散的方法通常需要标签或特定的溶剂.

研究的目的:

  • 开发一种新的分析技术,拉曼扩散顺序光谱学 (Raman-DOSY),它结合了尺寸和结构灵敏度.
  • 为了使扩散系数和水力动力学半径的光谱分辨率确定.
  • 为了证明Raman-DOSY用于分析复杂混合物的多功能性.

主要方法:

  • 拉曼扩散顺序光谱学 (Raman-DOSY) 使用微流体流细胞与样品和溶剂的平行层流进行.
  • 随着时间的推移,使用拉曼显微光谱技术监测了从样品流到溶剂流中的溶液扩散.
  • 产生了二维拉曼-DOSY光谱,将拉曼频率与扩散系数绘制成图.

主要成果:

  • 拉曼-多西成功地从不同大小的分子中分辨了重叠的拉曼峰.
  • 扩散系数和水力动力半径准确地导出了小分子,蛋白质和超分子组件 (小粒).
  • 该方法在分析含有多达三种化合物的混合物方面表现出了多功能性.

结论:

  • 拉曼-DOSY是一种强大的,无标签的技术,它集成了结构和尺寸信息.
  • 这种方法克服了现有技术的局限性,因为不需要化溶剂.
  • 拉曼-多西为分析多样样样本提供了一种多功能方法,包括那些对其他基于扩散的方法具有挑战性的样本.