在训练有素的跑步者中,对长期高空暴露的全血和外围单核细胞转录反应
Shaun Sutehall1, Fernanda Malinsky2, Jonathan Shurlock3
1Division of Physiological Sciences, University of Cape Town, Cape Town, South Africa.
概括
这项研究确定了运动员暴露在高海拔地区的独特基因表达模式. 这些发现有助于区分高度效应与体育运动中的复合人体红蛋白 (rHuEpo) 兴奋剂.
科学领域:
- 运动科学 运动科学 运动科学
- 分子生物学分子生物学
- 反兴奋剂研究研究
背景情况:
- 运动员滥用复合人体红素 (rHuEpo) 对体育诚信构成重大挑战.
- 由于重叠的血液学变化,区分rHuEpo影响与对高度暴露的生理反应是很困难的.
- 探索分子方法对于加强反兴奋剂策略至关重要.
研究的目的:
- 调查精英运动员长时间暴露在高空的血液学和转录学反应.
- 为了确定分子标记,可以区分高度适应和兴奋剂.
主要方法:
- 一项纵向研究涉及14名训练有素的运动员在高海拔训练营 (2400-2500米) 进行了27天.
- 在海拔暴露期间和返回海平时在多个时间点收集的血液样本.
- 在全血和PBMC中使用微阵列分析血红蛋白,血红素,网细胞百分比和基因表达.
主要成果:
- 全血中的29个基因和PBMC中的10个基因在高度暴露期间一致表达.
- 在全血中恢复到海平面时没有观察到显著的基因表达变化,而PBMCs中只有一个基因.
- 这些已识别的基因代表了高度适应的独特的转录基因特征.
结论:
- 该研究成功地确定了与长时间高空暴露相关的独特基因表达特征.
- 这些发现为将新的分子标记物与现有的rHuEpo检测方法相结合提供了基础.
- 这一进步将提高运动员在自然高度效应和提高表现的药物滥用之间进行区分的能力.
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