相关实验视频

Updated: Jul 17, 2025

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

在严重喘的支气管上皮细胞中构建lncRNA-miRNA-mRNA调节网络:生物信息学研究

Mengzhen Fan¹, Wenjie Song^1,2, Zheng Hao^1,2,3

¹School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

|September 1, 2023

概括

这项研究揭示了严重喘 (SA) 支气管上皮细胞 (BECs) 中的一种新型lncRNA-miRNA-mRNA调节网络,识别了参与疾病发病的关键基因和途径.

科学领域:

* 呼吸系统医学呼吸系统医学
* 分子生物学 * 分子生物学
* 生物信息学是一门学科.

背景情况:

*喘是一种慢性呼吸道疾病,受环境因素和宿主相互作用的影响.
* 支气管上皮细胞 (BECs) 对于防御环境毒素至关重要,但它们在严重喘 (SA) 中的作用需要进一步阐明.
*长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 在SA病变发生过程中参与调节基因表达.

研究的目的:

* 阐明SA患者的BECs中的lncRNA-miRNA-mRNA调节网络.
* 识别SA中核心差异表达基因 (DEGs) 和枢纽基因.
* 探索在BECs中涉及SA病原发生的潜在信号通路.

主要方法:

*下载并分析了SA和健康对照 (HC) 的5个mRNA数据集从基因表达综合 (GEO) 数据库.
* 采用Venn图和强大的排名聚合 (RRA) 来识别DEG,然后对枢纽基因进行蛋白质-蛋白质相互作用 (PPI) 分析.
*利用miRDB,miRWalk,ENCORI和starBase v2.0数据库来预测miRNA-mRNA和lncRNA-miRNA相互作用,构建一个竞争的内源RNA (ceRNA) 网络.

主要成果:

*确定了四个核心DEG:CEACAM5,IL1R2,TFF3和VEGFA,表明SA和性别之间没有显著的关联.
* 丰富分析突出显示MAPK,Rap1,Ras,PI3K-Akt和信号通路是SA BEC的主要通路.
* 构建了一个lncRNA-miRNA-mRNA调节网络,识别了顶级ceRNA,包括MALAT1,XIST和has-miR-106a-5p.

结论:

*揭示了一种潜在的调节机制,涉及到BECs中的lncRNA-miRNA-mRNA网络,这些网络有助于SA.
*确定了可能作为SA的治疗点的关键基因和途径.
*为SA在支气管上皮层水平的分子病原发生提供了新的见解.

更多相关视频

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...