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Lauren T Gallagher¹, Clyde J Wright², Tanner Lehmann¹

¹Laboratory for Fetal and Regenerative Biology, Department of Surgery, University of Colorado Denver School of Medicine, Aurora, Colorado; Division of Pediatric Surgery, Children's Hospital Colorado, Aurora, Colorado.

The Journal of surgical research

|September 1, 2023

概括

失调的微RNAs (miRs) miR-15b和miR-146a与胎儿生长限制 (FGR) 有关. 上调的miR-15b会损害血管形成,而下调的miR-146a会促进FGR胎盘的炎症.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
发育生物学是发展生物学.

背景情况:

胎儿生长限制 (FGR) 的特点是血管生成受损和慢性炎症.
微RNAs (miRs) 是基因表达的关键调节者,影响各种生物过程.
特定 miRs 的调节失调可能会导致 FGR 的致病性.

研究的目的:

调查miR-15b和miR-146a在FGR病变发生中的作用.
确定这些miRs的改变表达是否与FGR中的血管生成和炎症受损有关.

主要方法:

在怀孕期间使用热量限制建立了FGR的小鼠模型.
从FGR和对照组收集了胎盘组织.
使用定量实时PCR比较小组之间的miR和mRNA表达水平.

主要成果:

FGR胎盘显示miR-15b的表达增加,与抑制的血管内皮生长因子alpha相关.
在FGR胎盘中,miR-146a被降低调节,导致增高的促炎媒介 (IL-6,IL-8,NFkB1) 和氧化应激标志物 (HIF-1α,SOD2,Nox2) 的水平.

结论:

在FGR中异常血管生成和慢性炎症与失调的miR-15b和miR-146a表达有关.
这些发现表明FGR中miR-15b和miR-146a的后转录调节作用.
该研究强调了这些miRs作为FGR的潜在治疗目标.

关键词:

血管新生的产生.细胞因子 (cytokines) 是一种细胞因子.胎儿生长限制胎儿生长限制微RNAs是一种微RNA.

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