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鉴定Brassica rapa BrEBF1同类物及其在冷信号中的表征

Wangze Wu¹, Haobo Yang², Juan Shen³

¹College of Life Sciences, Northwest Normal University, Lanzhou, 730070, China.

Journal of plant physiology

|September 1, 2023

概括

布拉西卡的EIN3结合F-box 1 (EBF1) 通过调节活性氧物种 (ROS) 和激活MPK3/MPK6激酶活性来增强耐寒性. 这项研究澄清了EBF1的EBF1.

科学领域:

植物科学植物科学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

众所周知,EIN3结合的F-box 1 (EBF1) 能够影响Arabidopsis的寒冷耐受性.
在Brassica rapa中,EBF1在冷信号通路中的确切功能尚不清楚.

研究的目的:

研究EBF1同类在Brassica rapa对寒冷应激反应中的作用.
为了阐明EBF1介导的B. rapa.寒冷耐受性背后的分子机制.

主要方法:

在Brassica物种中对EBF1同类的比较分析.
在B. rapa品种的冷处理下进行基因表达分析.
BrEBF1.1.的亚细胞局部化和蛋白质降解研究.
通过过度表达,对Arabidopsis中的BrEBF1进行功能分析.

主要成果:

虽然EBF1同类被保存,但在Brassica物种中显示出不同的副本数和图案.
冷处理对B. rapa品种的BrEBF1和BrEBF2表达有不同的影响.
一种核蛋白BrEBF1在暴露在寒冷中经历了降解.
在Arabidopsis中BrEBF1的过度表达改善了耐寒性,减少了ROS,并提高了MPK3/MPK6活性.

结论:

BrEBF1积极调节了Brassica rapa的寒冷耐受性.
BrEBF1介导的寒冷耐受性包括反应性氧物种 (ROS) 清除和通过C重复结合因子通路激活MPK3/MPK6.

关键词:

BrEBF1 BrEBF1 的意思是什么黄铜 (Brassica rapa) 是一种葡萄.寒冷的压力是冷的压力.MAPK 激酶活性激酶的活性.在ROS清理垃圾.

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