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优化和内部验证的分析程序,用于官方控制的顿石作为亚拉托克素不活化剂的分析程序

Vito D'Ascanio¹, Donato Greco¹, Mariagrazia Abbasciano¹

¹Institute of Sciences of Food Productions (ISPA), National Research Council (CNR), 70126 Bari, Italy.

|September 1, 2023

概括

一个新的协议改善了对托尼特料添加剂的亚拉托克素结合能力 (BC_AfB1) 测试. 这样可以确保更多的顿石符合欧盟关于减轻黄毒素的标准,提高料安全.

科学领域:

动物营养动物营养
食品安全食品安全
矿物学是什么?矿物学是什么?

背景情况:

班托尼特是欧盟批准的用于减轻 aflatoxin (1m558) 的料添加剂,如果它们符合特定的矿物质学标准,并表现出高的 aflatoxin结合能力 (BC_AfB1 > 90%).
确定BC_AfB1的官方方法涉及吸附试验,但其稳定性尚未先前评估.
当前方法的局限性可能会阻止符合规范的顿石进入市场.

研究的目的:

调查官方方法的稳定性,以确定本托尼特的亚拉托克辛结合能力 (BC_AfB1).
为BC_AfB1评估开发和验证一个新的,改进的协议.
确保准确和可靠的评估本顿石的欧盟市场批准.

主要方法:

在六个矿物学上合规的顿石样本上进行了强度研究,测试因素包括悬浮制备,残留酸,缓冲度,化时间和离心.
开发了一个新的协议,保持官方测试的关键参数,然后在内部验证.
同温方法,比较兰木尔吸附参数与BC_AfB1值,用于证明新协议的有效性.

主要成果:

稳定性研究表明,残留的乙和离心法显著影响了官方测定结果.
官方方法错误地排除了6种本顿石中的4种 (BC_AfB1 <90%).
新开发的协议成功地为所有测试的顿石产生了BC_AfB1>>90%,显示出高精度 (RSD_I=3.4%) 和可靠性 (HorRat <2).

结论:

官方的测定班托尼特BC_AfB1的方法不稳定,导致符合规定的产品可能被排除在外.
新开发和验证的协议提供了一种可靠和精确的方法来评估青素结合能力.
这种改进的协议有助于准确评估顿石对阿弗拉托克辛的缓解作用,支持它们作为料添加剂的安全使用.

关键词:

吸附方式吸附方式吸附方式非洲毒素 (Aflatoxins) 是一种毒素.分析化学方法分析化学方法动物料是动物料.托尼特 (Bentonite) 是一种托尼特.安全的安全的安全的安全的安全.

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