MALDI-ToF MS和化学测量分析作为识别野生和养殖鱼的工具
Yunhe Hong1, Nicholas Birse1, Brian Quinn1
1National Measurement Laboratory, Centre of Excellence in Agriculture and Food Integrity, Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, United Kingdom.
Food chemistry
|September 1, 2023
概括
矩阵辅助激光脱吸电离时间飞行质谱学 (MALDI-ToF MS) 通过化学测量准确区分野生和养殖鱼. 这种方法为检测鱼产品中的食品欺诈提供了可靠的工具.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 区分野生和养殖的鱼对于食品的真实性和防止欺诈至关重要.
- 目前的方法可能缺乏准确性,以可靠地区分三文鱼生产类型.
- 矩阵辅助激光消耗离子化飞行质谱时间 (MALDI-ToF MS) 提供高通量蛋白质组分析.
研究的目的:
- 开发和验证一种使用MALDI-ToF MS.区分野生和养殖鱼的方法.
- 应用化学测量分析来对鱼样品进行可靠的分类.
- 评估MALDI-ToF MS在检测鱼欺诈方面的潜力.
主要方法:
- 采用矩阵辅助激光吸附离子化-飞行质谱时间 (MALDI-ToF MS) 进行样本分析.
- 采用多变量分析技术,包括主要成分分析 (PCA),部分最小平方区分分析 (PLS-DA) 和直角部分最小平方区分分析 (OPLS-DA).
- 验证了化学测量模型的分类和识别准确性.
主要成果:
- 通过化学模型,在区分养殖和野生鱼方面实现了100%的分类准确性.
- 证明了100%的识别准确性,用于区分野生Salmo salar和Oncorhynchus nerka样本.
- 确定了区分鱼生产类型的独特蛋白质特征.
结论:
- 拟议的MALDI-ToF MS方法与化学测量相结合,在区分野生和养殖鱼方面非常有效.
- 这种方法为检测与生产来源相关的鱼欺诈提供了可靠的参考.
- 该研究扩大了MALDI-ToF MS技术在食品真实性和质量控制方面的应用.
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