在黄色鼓 (Nibea albiflora) 中鉴定加列9及其抗菌功能
Shuai Luo1, Baolan Wu2, Qiaoying Li2
1Strait Institute of Flexible Electronics (SIFE, Future Technologies), Fujian Normal University, Fuzhou, 350117, Fujian, China; Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural, Fisheries College, Jimei University, Xiamen, 361021, China.
Fish & shellfish immunology
|September 1, 2023
概括
来自Nibea albiflora的YdGal-9蛋白显示出抗菌特性,对于对抗Vibrio harveyi感染至关重要. 这种加勒-9变体在鱼类中起着至关重要的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 海洋生物学 海洋生物学
- 生物化学 生物化学
背景情况:
- 盖莱克是保存的莱克,可以识别β-galactoside.
- 加列-9在感染期间对宿主防御至关重要.
- 之前的GWAS在黄色鼓中确定了 galectin-9 作为Vibrio harveyi疾病的抵抗基因.
研究的目的:
- 为了识别和描述来自Nibea albiflora (YdGal-9) 的 galectin-9.
- 为了研究YdGal-9在抗菌防御中对Vibrio harveyi的作用.
主要方法:
- 在N. albiflora组织中进行基因鉴定和mRNA表达分析.
- YdGal-9-EGFP融合蛋白的亚细胞局部化.
- 使用纯化重组YdGal-9及其突变的细菌聚合试验.
- 抗菌活性测定对各种格兰阴性细菌的抗菌活性.
主要成果:
- 在所有组织中都发现了YdGal-9mRNA,在脏中最高.
- YdGal-9局部化到细胞核和细胞质;其表达在Vibrio harveyi感染后的大脑中增加.
- 重组YdGal-9凝结红细胞和表现出对格兰氏阴性细菌的杀菌活性,保存的动机是关键的.
- 在V. harveyi,P. plecoglossicida和E. coli等细菌中,YdGal-9诱导细胞壁的破坏.
结论:
- YdGal-9 是 N. albiflora. 的天生的免疫系统的一个关键组成部分.
- YdGal-9表现出直接的抗菌活性,对于对抗致病性感染如Vibrio harveyi疾病至关重要.
相关概念视频
01:26Differential Staining Technique
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
01:13Special Staining Techniques
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
01:22Bacterial Cell Wall
The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
01:26Bacterial Phylum Proteobacteria
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
01:26Bacterial Phylum Bacteroidota
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...