对于核桃炭病原体Ophiognomonia leptostyla的参考转录组指导了候选毒性基因的发现
Fatemeh Khelghatibana1, Mohammad Javan-Nikkhah2, Naser Safaie3
1Department of Plant Pathology, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
Fungal genetics and biology : FG & B
|September 1, 2023
概括
这项研究介绍了Ophiognomonia leptostyla的第一个转录组组件,这是一种导致核桃炭菌的真菌. 它识别了候选病毒性基因,包括作用因子和碳水化合物活性酶,对于理解真菌感染策略至关重要.
科学领域:
- 植物病理学 植物病理学
- 菌类基因组学 菌类基因组学
- 分子生物学分子生物学
背景情况:
- 核桃炭,由Ophiognomonia leptostyla引起,导致了重大的经济损失.
- 目前O. leptostyla的基因组资源缺乏,这阻碍了对其致病性的研究.
研究的目的:
- 为了生成O. leptostyla. 的第一个de novo转录组组件.
- 为了确定候选毒性基因,包括碳水化合物活性酶 (CAZys) 和效应因子,参与核桃感染.
- 在真菌与宿主相互作用期间分析基因表达模式.
主要方法:
- 在注射后的多个时间点对O. leptostyla mycelia和受感染的核桃叶子进行RNA测序.
- 对新的转录组组装方法进行比较分析 (Trinity,Oases,SOAPdenovo-Trans,Bridger).
- K-意味着转录的集群,以识别时间表达模式.
- 在分析以确定候选效应蛋白和CAZys.
主要成果:
- 布里杰组件被确定为高级参考转录组.
- 在不同感染阶段观察到四种不同的转录模式.
- 在注射后96小时,CAZy转录的转录升高,与细胞内生长相吻合.
- 确定了103个候选效应因子,其中包括6个结核和乙烯诱导类似蛋白 (NLP).
结论:
- 这项研究为O. leptostyla提供了宝贵的基因组资源.
- 时间表达模式的CAZys和效应器提供了洞察到核桃炭病原体.
- 已识别的候选病毒性基因需要进一步验证,以了解O. leptostyla感染机制.
关键词:
凯西是什么意思 凯西候选效应因子的候选者从新开始,转录组组合组合.皇家子 (英语:Juglans regia,英语:Juglans regia,英语:Juglans regia,英语:Cv. (钱德勒) 钱德勒) 钱德勒) 钱德勒) 是一个K-表示集群.这种植物名为Ophiognomonia leptostyla.在RNA-seqqq.更多相关视频
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