基于网络药理学,分子对接和实验研究的Kedaling药片治疗动脉样硬化的作用和机制
Hongzheng Li1, Wenwen Yang2, Weiyi Cao2
1Xiyuan Hospital, China Academy of Chinese Medical Science, Beijing, 100091, China; Beijing University of Chinese Medicine, Beijing, 100029, China.
凯达林片 (KDL) 通过减少斑块形成和抑制关键炎症通路,有效治疗小鼠动脉样硬化 (AS). 这项研究确定了KDL.
科学领域:
- 药理学和中国传统医学
- 心血管研究研究心血管研究
- 动脉样硬化病原体的产生
背景情况:
- 来自Corydalis yanhusuo的Kedaling药片 (KDL) 是一种用于预防动脉样硬化 (AS) 的传统中医药.
- 了解KDL在AS治疗中的治疗作用和机制对于其临床应用至关重要.
研究的目的:
- 评估KDL在治疗AS中的疗效.
- 为了识别KDL片中的活性成分.
- 分析KDL治疗的老鼠血清参数的变化,阐明KDL在AS中的作用机制.
主要方法:
- 动脉样硬化诱导在ApoE淘汰赛小鼠中,这些小鼠被食高脂肪饮食.
- 用KDL或阿托瓦斯塔丁治疗小鼠;通过Movat和HE染色评估大动脉斑块.
- 分析了血清脂质概况,炎症因素和KDL成分 (UPLC-Q/TOF-MS);网络药理学和分子对接探索了机制.
主要成果:
- 在ApoE-/-小鼠中,KDL治疗显著减少了主动脉斑块面积,泡细胞和原纤维.
- KDL调节了总胆固醇,甘油三,HDL-C,LDL-C和关键炎症因子 (IL-1β,IL-17) 的血清水平.
- 网络药理学确定了255个潜在的标和177个信号通路 (包括TNF和TGF-β);分子对接证实了活性成分与TNF和TGF-β标的亲缘关系.
结论:
- 凯达林片在小鼠模型中显示出显著的抗动脉样硬化作用.
- KDL的治疗作用与通过TNF和TGF-β途径抑制炎症信号相关.
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