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血清来源对细胞因子的影响诱导了杀手细胞的扩张和功能

Zahra Jabbarpour¹, Seyed Sajjad Aghayan¹, Kobra Moradzadeh¹

¹Gene Therapy Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Shariati Hospital, North Kargar Ave, Tehran, 14117, Iran.

|September 1, 2023

概括

人类衍生的血小板溶解剂 (hPL) 在10%度显著增强细胞因子诱导的杀手细胞 (CIK) 扩张和功能,与胎儿牛血清 (FBS) 和人血清 (HS) 相比. 这一发现支持细胞治疗的良好生产规范指南.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

细胞因子诱导杀手 (CIK) 细胞对于采用免疫疗法至关重要.
血清来源和度影响临床用途的CIK细胞扩张.
良好的制造实践 (GMP) 准则鼓励在细胞疗法中减少动物产品.

研究的目的:

研究血清来源和血清度降低对CIK细胞扩张和功能的影响.
为了比较人类血小板溶解酸 (hPL),胎儿牛血清 (FBS) 和人类血清 (HS) 在CIK细胞培养中的疗效.
为了确定大规模CIK细胞扩张的最佳条件.

主要方法:

使用CIK细胞扩张协议培养了周围血液单核细胞 (PBMC).
细胞补充了hPL,FBS或HS,其度为10%,5%和2.5%.
扩散动力学在30天内进行监测.

主要成果:

在所有测试的血清中,10%的血清度显示出较高的细胞增殖比较低的度.
与FBS和HS相比,hPL在所有度中显著增加了扩张率.
接受hPL治疗的细胞表现出增强的活力,细胞毒性和CIK CD标志物表达.

结论:

10%的hPL对CIK细胞的增殖和功能表现出最有利的结果.
对于 CIK 细胞扩张,hPL 是 FBS 和 HS 的优质替代品.
优化血清条件对于临床CIK细胞治疗至关重要.

关键词:

细胞扩张细胞扩张细胞疗法是一种细胞疗法.细胞因子诱导的杀手细胞 (CIK)良好的制造实践 (GMP) 是一个

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