在结有机框架中共同封装辅助因子和酶,用于与辅助因子回收的多酶级联反应
1Department of Biochemical Engineering, School of Chemical Engineering and Technology and Key Laboratory of Systems Bioengineering and Frontiers Science Center for Synthetic Biology, Ministry of Education), Tianjin University, Tianjin, 300350, P. R. China.
Angewandte Chemie (International ed. in English)
|September 2, 2023
概括
一种新的多电解质辅助封装方法 (PAEA) 能够在结有机框架 (HOF) 中有效地联合固定酶和辅助因子. 这种方法增强了生物纳米反应器的酶稳定性和级联活性.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 在键有机框架 (HOF) 中的酶固定对活性恢复和依赖辅因子的多酶系统提出了挑战.
- 开发强大高效的酶和辅助因子联合不动化的方法对于先进的生物催化剂至关重要.
研究的目的:
- 开发一种新的多电解质辅助封装方法 (PAEA) 用于制造使用HOFs的辅助因子依赖的多酶级联纳米反应器.
- 克服酶活性恢复和HOFs内部复杂的多酶系统组装的局限性.
主要方法:
- 利用多电解质辅助封装方法 (PAEA) 在BioHOF-1中共同封装四个氧化还原酶和两个尼古丁胺氨酸二核酸 (酸盐) (NAD(P) H) 辅因子.
- 采用多电解质来覆盖酶和结NAD (P) H,促进与HOF单体的相互作用,同时保护生物分子.
- 展示了使用HOF-101创建多功能生物纳米反应器的方法.
主要成果:
- 在设计的BioHOF-1纳米反应堆中实现了优异的载荷和超过100%的级联活动.
- 多电解质有效地保护了酶和辅助因子免受HOF单体的影响,保持了它们的完整性和功能.
- 该PAEA方法导致固定酶和NAD(P) H辅因子的稳定性和可回收性得到改善.
- 成功制造了一个基于HOF-101的生物纳米反应器,展示了PAEA的多功能性.
结论:
- 聚电解质辅助封装方法 (PAEA) 为构建具有HOF的先进辅助因子依赖的多酶级联纳米反应器提供了一个简单和多功能策略.
- 在基于HOF的生物催化系统中,PAEA显著改善了酶活性恢复,稳定性和辅助因子管理.
- 这项研究为设计下一代酶性纳米反应器提供了一个有希望的平台,用于各种生物技术应用.
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