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使用FACS-FISH的血细胞FISH的优异检测率

Marie-France Gagnon¹, Sally M Midthun¹, James A Fangel¹

¹Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, Mayo Clinic, Rochester, MN, US.

American journal of clinical pathology

|September 2, 2023

概括

与细胞质免疫球蛋白 FISH (cIg-FISH) 相比,光激活细胞分类 FISH (FACS-FISH) 对血细胞瘤 (PCN) 提供了更好的诊断产量. FACS-FISH提高了检测率,并减少了临床环境中的工作流要求.

科学领域:

血液学血液学血液学
细胞遗传学细胞遗传学
分子诊断学分子诊断

背景情况:

血细胞瘤 (PCN) 的诊断依赖于FISH,需要有效的血细胞 (PC) 识别或净化.
目前的方法,如细胞质免疫球蛋白 FISH (cIg-FISH),在优化结果方面存在局限性.

研究的目的:

为了在临床环境中比较cIg-FISH和光激活细胞分类FISH (FACS-FISH) 对PCN的诊断疗效.
评估FACS-FISH对检测率,工作流程和成本效益的影响.

主要方法:

分析了从2019年至2022年期间对14855个疑似PCN的FISH测试.
与cIg-FISH (n=6917) 和FACS-FISH (n=7938) 的结果进行比较.
评估特定的遗传异常和PC产量.

主要成果:

与cIg-FISH相比,FACS-FISH显示出更高的异常检测率 (54%与50%相比,P<.001).
FACS-FISH显著改善了IGH::CCND1,IGH::MAF,IGH::MAFB,其他IGH重排的检测,以及1q增益/放大.
鱼类的PC收益率不足从22% (cIg-FISH) 降至3% (FACS-FISH),在91%的案例中确定了性状态.

结论:

FACS-FISH是PCN的卓越PC净化策略,显著提高了诊断产量.
与cIg-FISH相比,FACS-FISH简化了工作流程,减少了分析时间,并降低了运营成本.

关键词:

这就是FACS-FISH.对鱼类进行测试.鱼是一种鱼.细胞质免疫球蛋白 FISH 光激活细胞分类 FISH 多发性骨髓瘤是多发性骨髓瘤的一种.血细胞瘤的发生.

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FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

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