乳酸细菌分泌的蛋白质作为潜在的Listeria monocytogenes 质量检测抑制剂
Pedro Henrique Marques1, Arun Kumar Jaiswal1, Felipe Alves de Almeida2
1Interunit Bioinformatics Graduate Program, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Molecular diversity
|September 2, 2023
概括
乳酸细菌分泌蛋白质,可以破坏Listeria monocytogenes的通信. 这些发现为控制李斯特菌感染和在食品环境中的持久性提供了一个有前途的反定数感应策略.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 计算生物学 计算生物学
背景情况:
- 李斯特菌 (Listeria monocytogenes) 引起李斯特菌病,并通过形成生物膜在食品环境中持续存在,这一过程由定数感应 (QS) 调节.
- 针对QS系统提供了一个有希望的策略来控制L. monocytogenes的毒性和持久性.
研究的目的:
- 通过in silico方法识别乳酸细菌 (LAB) 中分泌的蛋白质,这些蛋白质可以抑制L. monocytogenes的农业QS系统.
- 探索用于控制L. monocytogenes的新型抗QS策略.
主要方法:
- 采集Lacticaseibacillus rhamnosus GG和Lactobacillus acidophilus NCFM的基因组,以确定分泌的蛋白质.
- 在L. monocytogenes AgrB和AgrC蛋白质结构的in silico建模和验证.
- 蛋白质-蛋白质对接和分子动力学模拟以评估LAB蛋白和L. monocytogenes QS组件之间的相互作用.
主要成果:
- 从LAB中分泌的151种蛋白质被确定.
- 血清类型的D-Ala-D-Ala碳酸酶 (来自L. rhamnosus GG) 和L,D-转酶 (来自L. acidophilus NCFM) 对L. monocytogenes的AgrB和AgrC蛋白分别表现出很高的亲和力.
- 这些LAB蛋白具有抑制循环自诱导转位和识别的潜力,从而破坏了农业质量系统.
结论:
- 分泌的LAB蛋白质,特别是血清类型的D-Ala-D-Ala碳酸酶和L,D-转酶,显示出作为L. monocytogenes农业QS系统的抑制剂的潜力.
- 这项研究提出了一个有前途的在体中识别的抗QS策略,用于控制L. monocytogenes在食品加工环境中.
- 这些发现可能会导致新的辅助疗法,用于治疗李斯特菌病.
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