在中国传统医学中,使用metabarcoding与特定的迷你条形码相结合,对lonicerae japonicae flos进行定性识别
Yuan Gao1,2, Xiaoying Zhang1,2, Wenxiu Wang1,2
1State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.
Molecular biology reports
|September 2, 2023
概括
一种新的DNA迷你条形码方法可以准确地识别传统中医药中的Lonicerae japonicae flos (Jinyinhua). 这种DNA元编码方法提高了质量控制,并通过检测Jinyinhua产品中的伪造来确保消费者的安全.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分子生物学分子生物学
- 传统中国医药 传统中国医药
背景情况:
- 龙 (Jinyinhua,JYH) 是中国传统专利药 (TCPM) 的一个关键成分.
- 使用劣质材料的改和替代对消费者安全构成风险.
- 在TCPM中准确识别JYH的物种对于质量控制至关重要.
研究的目的:
- 在TCPM中开发一种可靠的方法来识别JYH物种组成.
- 为了解决物种级别识别的通用ITS2开头的局限性.
- 建立一种DNA元编码方法,用于对JYH进行定性和定量分析.
主要方法:
- 最初使用了通用ITS2原料,但无法得出确的物种级结果.
- 开发了一种针对psbA-trnH区域的新型迷你条形码启动器.
- 为了分析,DNA元条码技术与迷你条码相结合.
主要成果:
- 迷你条形码启动器,与DNA元编码,使定性和定量分析.
- 该方法成功地区分了含有JYH的TCPM.
- 在混合样本中证明了两个Lonicera物种的准确定量识别.
结论:
- 建立了一种强大的定性和定量JYH识别方法.
- DNA元编码技术对TCPM质量控制有显著的前景.
- 这种方法提高了传统中医药的安全性和可靠性.
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