寒冷压力通过DUSP-JNK通路诱导银的亡
Jiabao Hu1,2,3,4, Man Zhang1,3,4, Kaiheng Yan1,3,4
1College of Marine Sciences, Ningbo University, Ningbo, China.
Marine biotechnology (New York, N.Y.)
|September 2, 2023
概括
寒冷压力会诱导肌肉纤维退化和白银的亡. 虽然DUSP最初抑制了细胞亡,但长时间暴露于寒冷会对JNK-C-JUN进行上调,导致细胞死亡增加.
科学领域:
- 水生动物生理学 水生动物生理学
- 分子生物学分子生物学
- 鱼类应激反应的压力反应
背景情况:
- 寒冷压力是鱼类水产养殖的重大环境挑战.
- 了解寒冷应激反应的分子机制对于鱼类的健康和生存至关重要.
- 银 (Pampus argenteus) 是一种经济重要鱼类,容易受到温度波动的影响.
研究的目的:
- 为了研究水温下降对银肌肉的影响.
- 为了确定关键的基因和途径参与鱼类冷诱导的亡.
- 阐明DUSP和JNK-C-JUN通路在鱼类寒冷应激反应中的作用.
主要方法:
- 银在降温状态下 (18°C至8°C) 培养了20天.
- 肌肉组织使用血素和埃 (HE) 染色,TUNEL测定和转录组分析进行了分析.
- 与亡相关的基因的基因表达通过RT-qPCR在鱼肌和培养的PaM细胞中得到量化.
主要成果:
- 肌肉纤维退化和亡在13°C以下显著增加.
- 转录组分析显示了与亡相关的途径的丰富.
- 关键的亡相关基因 (JNK1,PIDD,CytC,Casp 3,GADD45) 在寒冷压力下被上调,而其他基因 (DUSP3,JNK2,PARP) 在寒冷压力下被下调. DUSP5显示了复杂的调节,最初在临界低温下升调,然后降调. 在较低的温度下,JNK-C-JUN通路的激活与亡的增加相关.
结论:
- 寒冷压力通过激活特定的分子通路诱导肌肉亡.
- DUSP可能在早期的寒冷压力中起到抑制作用,但其在低温下降调,加上JNK-C-JUN激活,促进了亡.
- 这些发现提供了关于鱼类寒冷压力机制的见解,以及改善水产养殖复原力的潜在目标.
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