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在骨关节炎的发展过程中,SNORC Knockdown可以缓解炎症,自缺陷和子细胞的矩阵降解

Zhifang Tang¹, Hanzhen Feng¹, Xusheng Chen²

¹Clinical Medical College of Dali University, Dali, 671000, China.

Molecular and cellular biochemistry

|September 2, 2023

概括

在骨关节炎 (OA) 中,丰富于软骨的小小新型 (SNORC) 是上调调的. 减少SNORC可以通过抑制PI3K和JNK通路来缓解OA炎症,矩阵降解和胆细胞亡.

科学领域:

生物化学生化学
分子生物学分子生物学
病理学病理学病理学

背景情况:

骨关节炎 (OA) 涉及过度的软骨细胞炎症和自功能受损.
识别像SNORC这样的关键分子参与者对于理解OA病变发生至关重要.

研究的目的:

为了调查小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的小的
阐明SNORC影响OA炎症,矩阵降解和自的分子机制.

主要方法:

对GEO数据集的生物信息分析 GSE207881.1.
定量实时PCR,免疫组织化学染色和OA大鼠模型和IL-1β刺激的胆红细胞中的西部涂抹.
基因淘汰实验评估SNORC对状细胞行为和信号通路的功能影响 (PI3K/AKT,JNK/c-Jun).

主要成果:

在OA软骨和IL-1β刺激的红细胞中,SNORC显著上调.
在SNORC Knockdown中抑制了炎症媒介 (NO,IL-6,TNF-α,PGE2),降低了矩阵降解标记 (ADAMTS5,MMP13) 和增加了2型原蛋白 (COL2A1).
降低SNORC调节恢复了自,缓解了线粒体功能障碍和亡,并抑制了PI3K/AKT和JNK/c-Jun信号传递.

结论:

SNORC是通过促进炎症,矩阵降解和软质细胞的自缺陷来促进OA病原发生的关键因素.
针对SNORC,可能通过其调节PI3K和JNK通路,为骨关节炎提供治疗策略.

关键词:

自自是一种自的过程.冠状细胞 (chondrocytes) 是一种细胞.细胞外矩阵降解细胞外矩阵降解骨关节炎是一种骨关节炎.丰富于软骨的小小说

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