在Rim4-mRNA相互作用的自介导监测中,有计划的介质翻译保护措施
Rudian Zhang1, Wenzhi Feng1, Suhong Qian1
1Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Cell reports
|September 2, 2023
概括
自在酵母杂化过程中选择性地降解RNA结合蛋白Rim4,释放其结合的mRNA用于翻译. 这一过程由Rim4规范.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 自在酵母半转化过程中至关重要.
- 化特异性RNA结合蛋白 (RBPs) 调节基因表达.
- 没有完全理解RBPs的作用和它们在变过程中与自的相互作用.
研究的目的:
- 为了研究在酵母菌中介性自过程中对化特异性RBP Rim4及其相关mRNA的命运.
- 阐明自细胞调节Rim4及其绑定转录的机制.
- 确定Rim4在化过程中激活选择性自的作用.
主要方法:
- 对Rim4.的核定位信号 (NLS) 分析.
- 在介质性自过程中研究Rim4-mRNA复合体命运.
- Atg11-依赖的降解试验.
- 在体外激酶测试中使用纯化的Rim4和Atg1激酶复合物.
主要成果:
- Rim4通过NLS进入细胞核,结合mRNA,并被输出到细胞质.
- 起初,Rim4-mRNA复合体被自性节省,但Rim4随后以Atg11依赖的方式降解.
- 释放的mRNA变得易受自,Rim4通过其RNA识别动机 (RRMs) 激活Atg1激酶.
结论:
- 自选择性降解Rim4在半分裂过程中,使其目标mRNAs的特定阶段翻译.
- Rim4的RRMs参与激活Atg1激酶,促进选择性自.
- 自监测Rim4-mRNA相互作用以控制介质翻译时间.
关键词:
在Atg1上在Atg1111上CP: 细胞生物学 细胞生物学这里是 Pab1 Pab1 .在RBP中使用RBP.边缘4 边缘4 边缘4mRNAs 是一个mRNAs.介质变化 (meiosis) 是一种变质的过程.一个核的核.选择性自自是一种选择性自.更多相关视频
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