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Updated: Jun 4, 2026

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高通量半自动无标签或基于TMT的高通量半自动化基蛋白质形分析协议

Claire Koenig1, Ana Martinez-Val1, Previn Naicker2

  • 1Novo Nordisk Foundation Center for Protein Research, Copenhagen, Denmark.

STAR protocols
|September 2, 2023
PubMed
概括

我们为Tandem Mass Tags数据依赖采集 (TMT-DDA) 和无标签量化数据独立采集 (LFQ-DIA) 提供了一个模块化管道,以改进蛋白质组概况. 这份指南帮助研究人员选择最适合自己的工作流程.

关键词:
质谱测量质量谱测量蛋白质生物化学 蛋白质生物化学蛋白质组学是指蛋白质组学.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...