miRNAs:作为新生儿败血症诊断和预后工具的新型非侵入性生物标志物
Nashwa El-Khazragy1, Noura Mostafa Mohamed2, Mohamed Fathalla Mostafa3
1Department of Clinical Pathology-Hematology and Ain Shams Medical Research Institute (MASRI), Faculty of Medicine, Ain Shams University, Cairo, Egypt; Department of Genetics and Molecular Biology, Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt; Department of Medical Research, Armed Forces College of Medicine (AFCM), Cairo, Egypt.
Diagnostic microbiology and infectious disease
|September 2, 2023
概括
三种特定的microRNAs (miRNAs) 显示为早期新生儿败血症 (NS) 的生物标志物具有前途. 升高的miR-1和miR-124水平表明NS,而降低的miR-34a则有助于诊断和预测结果.
科学领域:
- 分子生物学分子生物学
- 新生儿医学 新生儿医学
- 生物标志物发现发现
背景情况:
- 新生儿败血症 (NS) 是一种严重的疾病,需要准确的早期诊断.
- 微RNA (miRNA) 正在成为各种疾病的潜在诊断和预后指标.
- 在早期NS中特定miRNAs的作用需要进一步调查.
研究的目的:
- 评估miR-1,miR-124和miR-34a在新生儿早期败血症中的诊断和预后价值.
- 识别潜在的miRNA生物标志物用于NS诊断和结果预测.
主要方法:
- 使用定量实时PCR (RT-PCR) 来测量miRNA表达水平.
- 在70名NS患者和70名健康对照中分析了miR-1,miR-124和miR-34a的表达.
- 进行统计分析以将miRNA水平与NS诊断和临床结果相关联.
主要成果:
- 在NS患者和对照人群之间观察到miRNA表达的显著差异.
- 较高的miR-1和miR-124水平与NS相关,而miR-34a水平则下降.
- miR-34a在确认NS方面表现出高特异性 (97%);miR-1和miR-124预测了疾病进展和死亡率.
结论:
- miR-1,miR-124和miR-34a显示出作为早期新生儿败血症的有价值生物标志物的潜力.
- 这些miRNA可以帮助诊断和预测新生儿败血症的临床结果.
- 需要进一步的研究来验证这些发现并探索临床应用.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...


