营养条件调节了Pseudomonas aeruginosa的抗生素耐受性
Huizhong Liu1, Yingsi Wang1, Zhiqing Zhang1
1Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
The Science of the total environment
|September 2, 2023
概括
营养素的可用性显著影响Pseudomonas aeruginosa的抗生素耐受性. 富含营养的条件增强了对某些抗生素的耐药性,而营养限制会导致休眠性和对其他抗生素的耐受性,这会影响各种环境中的抗生素疗效.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 分子生物学分子生物学
背景情况:
- 微生物的生存和新陈代谢受环境营养水平的影响.
- 了解细菌抗生素耐受性的营养介导变化对于预测治疗结果和耐药性发展至关重要.
研究的目的:
- 调查营养可用性对Pseudomonas aeruginosa PAO1.1.抗生素耐受性的影响.
- 阐明了营养受影响抗生素耐药性的基础分子机制.
主要方法:
- 在营养丰富和营养有限的条件下对P. aeruginosaPAO1抗生素耐受性的比较分析.
- 转录组分析以确定差异表达的基因.
- 使用酸盐干扰ATP合成的实验验证.
主要成果:
- 在营养丰富的介质中,P. aeruginosa对塞夫塔齐迪姆,氨基醇和四环素的耐受性增加,这与上调的核糖体蛋白和多药物排泄基因有关.
- 营养素的限制导致了转化,生物合成和氧化酸化基因的抑制,降低了代谢活性,增加了对西普罗夫洛克萨的耐受性.
- 与ATP合成的干扰证实了能量耗尽在增强西普洛素耐受性的作用.
结论:
- Pseudomonas aeruginosa 在营养丰富的环境中激活内在耐药性机制,增强对特定抗生素的耐受性.
- 在营养有限的条件下,P. aeruginosa进入休眠状态,增加对通常敏感的抗生素的耐受性.
- 抗生素的有效性和耐药性的发展可能会在不同营养水平的环境中显著变化 (化).
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