三环碳化合物烯减弱了内腔离子电流和压力发展在纳瓦加鱼
Denis V Abramochkin1, Tatiana S Filatova2, Vladislav S Kuzmin3
1Department of Biology, MSU-BIT University, Shenzhen, Guangdong Province, China; Department of Human and Animal Physiology, Lomonosov Moscow State University, Leninskiye Gory, 1, 12, Moscow, Russia.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
|September 2, 2023
概括
来自石油泄漏的多环芳 (PAH) 对北极鱼类有害. 是一种PAH,显著扰乱极地鱼心脏的电活动,特别是IKr电流,同时仅略有影响收缩功能.
科学领域:
- 环境毒理学环境毒理学
- 心血管生理学心血管生理学
- 北极海洋生物学 北极海洋生物学
背景情况:
- 石油泄漏释放多环芳 (PAH),对北极生态系统构成重大风险.
- 氧化会在鱼类中引起各种毒性作用,心脏功能障碍是主要关注的问题.
- 像纳瓦加鱼这样的极地鱼类对环境污染物特别脆弱.
研究的目的:
- 研究,一种常见的三环PAH,对极地纳瓦加鱼 (Eleginus nawaga) 的心脏电生理学和收缩性的影响.
- 为了确定主要离子电流 (IKr,INa,ICaL,IK1) 和动能波形对素暴露的灵敏度.
- 评估烯对孤立的纳瓦加鱼心脏的整体收缩功能的影响.
主要方法:
- 电生理学记录离子电流 (IKr,INa,ICaL,IK1) 和孤立心室肌细胞中的作用电位.
- 孤立的整个心脏中收缩活性的评估,这些心脏受到不同度的化物.
- 药理学分析包括IC50测定对IKr的影响.
主要成果:
- 烯对快速延迟整流器K+电流 (IKr) 具有很高的敏感性,其IC50为0.54μM.
- 进入的电流 (INa和ICaL) 在10μM中显示出中度的抑制 (20-28%) ,而IKr受到更强烈的影响.
- 作用电位的持续时间延长了3μM的,AP上升速度在10μM下降;收缩功能仅表现出适度的抑郁.
结论:
- 素显著影响极地鱼心脏的电活动,主要是通过影响IKr电流.
- 观察到的电生理学障碍表明,在暴露于烯的北极鱼类中,存在潜在的节律失常风险.
- 虽然电功能受到严重影响,但收缩活性仅受到中度影响,这表明这种PAH在极地鱼中具有复杂的毒理特征.
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