来自Alnus sieboldiana的NADPH依赖双键还原酶的克隆和表征,这些还原酶可以识别线性二甲基胺作为基质
Konosuke Takemoto1, Akiho Tsurugi-Sakurada2, Ryota Moriuchi3
1Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; The United Graduate School of Agricultural Science, Gifu University, 1-1, Yanagido, Gifu-shi, 501-1193, Japan.
Phytochemistry
|September 2, 2023
概括
研究人员在Alnus sieboldiana中发现了AsDBR1和AsDBR2两种酶,可以减少循环二甲基乙胺 (CDH). 这些发现揭示了CDHs的生物合成,CDHs是重要的植物二次代谢物.
科学领域:
- 植物生物化学 植物生物化学
- 酶学 是一种酶学.
- 二次代谢物生物合成二次代谢物生物合成
背景情况:
- 烯类化合物是植物二次代谢产物,具有C6-C7-C6支架,被分类为线性或循环.
- 循环二甲胺 (CDHs) 是由像Alnus这样的actinorhizal树产生的,并且与增加结节的形成有关.
- 关于CDH生物合成的信息仍然有限,特别是关于减小肝链的信息.
研究的目的:
- 确定参与Alnus sieboldiana周期性二甲基类 (CDHs) 生物合成的基因.
- 描述CDH合成中已识别的双键还原酶 (DBRs) 的功能和特性.
主要方法:
- 选Alnus sieboldiana基因与已知的植物双键还原酶相似.
- 两种特定酶AsDBR1和AsDBR2的分离和表征.
- 酶检测测以确定基质特异性,最佳pH值,对于bisdemethoxycurcumin和NADPH降解的动力参数 (Km,kcat).
主要成果:
- 两个Alnus sieboldiana双键还原酶,AsDBR1和AsDBR2,被分离和表征.
- 这两种酶都催化了 bisdemethoxycurcumin 和curcumin 的依赖NADPH 的减少.
- 在pH值5.0下观察到AsDBR1和AsDBR2的最佳活性,对bisdemethoxycurcumin和NADPH的动力参数 (Km,kcat) 是不同的.
- 对于 bisdemethoxycurcumin 减少,AsDBR1 的催化效率 (kcat) 比AsDBR2显著更高.
结论:
- AsDBR1和AsDBR2是减少Alnus sieboldiana中的特定循环二甲基乙胺的关键酶.
- 这些发现提供了关于和循环二甲基类药物的生物合成途径的见解.
- 这些已识别的酶有助于了解植物二次代谢产物形成的过程,这些代谢产物在共生中可能发挥作用.
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