阿拉伯氨基酸源和西兰酶的特异性影响了具有前生物潜力的寡糖类的产生
Reskandi C Rudjito1, Amparo Jiménez-Quero1, Maria Del Carmen Casado Muñoz1
1Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, AlbaNova University Centre, SE-106 91 Stockholm, Sweden.
Carbohydrate polymers
|September 2, 2023
概括
谷物阿拉比诺西兰 (AXs) 的结构影响着西兰酶活性,产生特定的寡糖化物 (AXOS). 这会影响肠道细菌和有益的短链脂肪酸生产.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 谷物阿拉比诺基兰 (AXs) 是复杂的多糖类,具有可变的阿拉比诺酸和酸替代.
- 这些替代物对结构和营养应用的AX特性产生了重大影响.
研究的目的:
- 研究小麦和麦AXs的分子结构如何影响各种β-xylanases的活性.
- 了解由此产生的阿拉比诺西兰-寡糖化物 (AXOS) 概况及其含义.
主要方法:
- 使用glycosyl hydrolase家族 (GH) 5_34,8,10和11中的β-xylanases进行AXs的酶性水解.
- 对AXOS产品的分析和基于AX替代模式的西兰酶特异性的表征.
主要成果:
- GH10 (Aspergillus aculeatus) 和GH11 (Thermomyces lanuginosus) 氧化酶表现出高活性,迅速产生了小的AXOS.
- GH8 (Bacillus sp.) 已经成为一种常见的药物. 产生的线性氧化酸糖,通过阿拉比诺斯替代而受到限制.
- GH5_34 (Gonapodya prolifera) 需要对活动进行阿拉比诺斯替代,产生更长的,替代的AXOS.
结论:
- 一个AX源和一个西兰酶的特异性决定了AXOS的结构.
- 特定的AXOS结构调节了肠道细菌的生长,比如Bacteroides ovatus和Bifidobacterium adolescentis.
- 这影响了有益的短链脂肪酸的产生.
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