电压调节的通道控制扩展访问可卡因寻找:核accumbens天体细胞的作用
Mengfan Xia1,2, Tanner L Anderson1, Emily R Prantzalos3
1Department of Neuroscience, University of Kentucky, Lexington, KY, USA.
概括
星体细胞在核贝表现出功能性可塑性通过电压通道 (Kv7). 在延长可卡因接入的老鼠中抑制这些通道减少了寻找毒品的行为,揭示了成中新的神经质机制.
科学领域:
- 神经科学是一个神经科学.
- 天星细胞生物学 天星细胞生物学
- 神经药理学神经药理学
背景情况:
- 核贝 (NAc) 中的多巴胺基信号传递对于奖励学习和可卡因行为至关重要.
- 恒星细胞,传统上被认为是不可兴奋的,对多巴胺和可卡因有结构性的反应,但它们的功能性可塑性仍未得到充分研究.
- 天体细胞电压通道活动的行为相关性,特别是在成中,在很大程度上是未知的.
研究的目的:
- 为了研究多巴胺或可卡因暴露后NAc天体细胞中通道的功能可塑性.
- 在可卡因自我管理的背景下,确定调节天体细胞电压门通道活动的行为影响.
- 阐明天体细胞Kv7通道在调节细胞内和寻找可卡因行为中的作用.
主要方法:
- 电生理学和Ca2+成像,以表征星体细胞通道功能和细胞内动态.
- mRNA表达分析 (Kcnq2) 和质谱测量以评估NAc中的分子变化.
- 行为研究涉及NAc向的Kv7通道抗剂和星球细胞毒素 (酸) 微注射在不同可卡因接入计划下的老鼠中.
主要成果:
- 外源性多巴胺增加了NAc天体细胞中的Kv7通道活性和Kcnq2转录水平.
- 扩大可卡因的使用导致NAc中多巴胺水平增加.
- Kv7通道抑制有选择地增强了星细胞Ca2+过渡体,并减弱了延长访问大鼠的可卡因寻找,这种效应被酸阻断.
结论:
- 在NAc天体细胞中,电压关闭的K+通道信号是行为相关的,并有助于寻找可卡因.
- Kv7通道调节天体细胞信号,这表明它在与药物成有关的神经质相互作用中发挥了作用.
- 这项研究揭示了在物质使用障碍的背景下,天体细胞功能可塑性的新机制.
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