MAVS集成了葡萄糖代谢和RIG-I类受体信号传递
Qiao-Qiao He1, Yu Huang1, Longyu Nie1
1State Key Laboratory of Virology, Modern Virology Research Center, Frontier Science Center for Immunology and Metabolism, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
Nature communications
|September 2, 2023
概括
线粒体抗病毒信号传递 (MAVS) 蛋白调节葡萄糖新陈代谢,通过将糖解向酸路径 (PPP) 和六胺生物合成路径 (HBP) 等路径转移. 过氧体和MAMs相关的MAVS在这种代谢重编程和干扰素信号传递中具有不同的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 代谢途径 代谢途径
背景情况:
- 线粒体抗病毒信号传递 (MAVS) 蛋白对于RIG-I类受体 (RLR) 信号传递至关重要.
- MAVS在葡萄糖代谢中的确切作用及其与RLR跨器官信号的交叉调节仍然不清楚.
- 了解这些通路是如何协调的,对于破译细胞防御机制至关重要.
研究的目的:
- 研究MAVS在葡萄糖代谢中的作用.
- 阐明RLR信号与葡萄糖代谢之间的交叉调节.
- 确定MAVS在协调这些途径中的器官特异性功能.
主要方法:
- 研究了MAVS在过氧体和线粒体关联ER膜 (MAM) 中的局部化和功能.
- 分析了使用代谢途径追踪的葡萄糖流量转移.
- 在使用共免疫沉和西式涂抹的MAVS信号体内确定了蛋白质-蛋白质相互作用.
主要成果:
- 通过MAVS激活RLR促进了从糖解转换到酸通路 (PPP) 和六胺生物合成通路 (HBP) 的代谢转换.
- 过氧体MAVS将葡萄糖流向PPP,并诱导III型干扰素的表达.
- 与MAMs相关的MAVS将葡萄糖流向HBP,并诱导I型干扰素的表达,涉及特定的蛋白质相互作用,如G6PD和谷氨酸-果糖-6-酸盐转氨酶.
结论:
- MAVS充当关键的调解者,将RLR信号与葡萄糖代谢联系起来.
- 特定于有机体的MAVS定位决定了不同的代谢和免疫信号结果.
- 这项研究揭示了一种新的新陈代谢重编程机制,在先天免疫反应期间由MAVS主导.
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