最丰富的深海异质型细菌可以通过培养来获取
Isabel Sanz-Sáez1, Pablo Sánchez2, Guillem Salazar3
1Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar, ICM-CSIC, 08003, Barcelona, Spain. isanz@icm.csic.
ISME communications
|September 2, 2023
概括
从深海培养的海洋细菌成功地恢复了比以前想象的更多的物种,特别是从沉没的颗粒中. 这些可培养的细菌是深海微生物群落的关键组成部分.
科学领域:
- 海洋微生物学 海洋微生物学
- 深海生态 深海生态
- 文化经济学就是文化经济学.
背景情况:
- 传统的培养技术只捕获了海洋微生物多样性的很小一部分,通常被称为稀有生物圈.
- 培养在代表深海微生物群落的有效性在很大程度上尚未在广泛范围内进行测试.
研究的目的:
- 调查在光层和深海层中通过培养依赖的方法可回收的异质性海洋细菌的比例.
- 将培养分离物与来自各种海洋深度的大规模16S rRNA基因元编码数据集进行比较.
主要方法:
- 来自2003年培养的异构性海洋细菌的16S rRNA基因序列与来自表面,半层和浴层样本的全球16S rRNA amplicon序列变体 (ASV) 的比较.
- 从全球元编码数据集中分析了60,322个独特的16S ASV,其中包括用于细菌分离的16个样本.
主要成果:
- 显著更高比例的培养分离与深海层的ASV相匹配,达到高达28%的浴微生物群落.
- 在前10个最丰富的浴类ASV中,三种属于Sulfitobacter,Halomonas和Erythrobacter,被成功分离出来.
- 培养分离物在海海洋 (38-45%) 的浮游生物大小部分中对原生生物群落做出了重大贡献.
结论:
- 浸沉的粒子在浴区作为营养丰富的息地,支持复制性,可培养的异构型细菌.
- 在深海中发现的可培养细菌也可以作为自由生活的生物存在和繁荣.
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