在xylamine应激下,在部分化/anammox系统中,anammox颗粒的破坏和重建
Wanyi Feng1, Junfei Qiao2, Jialin Li1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, PR China.
Journal of environmental management
|September 3, 2023
概括
氧胺压力导致anammox细菌 (AnAOB) 颗粒分解和重建,增强部分化/anammox (PN/A) 生物反应器中的去除. 这种自我重建过程提高了废水处理PN/A系统的可行性.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 生物反应器工程学
背景情况:
- 酸盐氧化细菌 (NOB) 往往超过氧化细菌 (AnAOB),阻碍了市政废水的部分化/氧化 (PN/A) 过程.
- 有效的PN/A实施需要管理NOB和AnAOB之间的竞争动态.
研究的目的:
- 调查氧胺 (NH2OH) 对PN/A生物反应器的影响,重点关注AnAOB弹性和自我重建.
- 了解AnAOB颗粒回收背后的机制及其对去除效率的影响.
主要方法:
- 基于颗粒的PN/A生物反应器运行了260天,间歇性添加NH2OH.
- 定量PCR (qPCR) 用于评估NOB种群和动态变化.
- 同焦激光扫描显微镜 (CLSM) 用于可视化颗粒结构.
- 分析颗粒的物理和形态特征.
- 通过RT-qPCR和高通量测序来研究功能性基因表达和微生物社区结构.
主要成果:
- 添加NH2OH导致NOB的延迟抑制,因为它们位于颗粒结构内.
- 阿纳莫克斯颗粒分解了,但随后被ANAOB用于重建新的颗粒.
- 观察到功能性基因表达和社区结构的显著变化,有利于AnAOB代谢.
- AnAOB的扩散率从0.52%增加到1.99%,去除率提高了近四倍 (0.21到0.83公斤-N/m3·d).
结论:
- 在PN/A系统中,anammox颗粒在NH2OH诱导的应力下表现出自我重建的能力.
- 这种弹性机制扩大了PN/A过程的可行性和应用潜力,以实现有效的废水处理.
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