FOS和JUN基因和FTIR光谱的表达数据提供了甲状腺癌的诊断
João Paulo da Silva Queiroz1, Breno Pupin1, Tanmoy Tapobrata Bhattacharjee2
1Laboratório de Biologia Molecular do Câncer, Universidade do Vale do Paraíba, UNIVAP, Instituto de Pesquisa e Desenvolvimento, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos, 12244-000 São Paulo, SP, Brazil.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|September 3, 2023
概括
这项研究表明,测量FOS和JUN基因表达和使用ATR-FTIR光谱学可以帮助诊断甲状腺癌. 这些方法对识别乳头状和卵泡状甲状腺癌有前途.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 频谱学是一种光谱学.
背景情况:
- 甲状腺癌的诊断依赖于准确的组织学评估.
- 分化甲状腺癌,如乳头甲状腺癌 (PTC) 和卵泡甲状腺癌 (FTC),存在诊断方面的挑战.
- 新的分子和光谱技术为改善诊断准确性提供了潜力.
研究的目的:
- 评估使用FOS和JUN基因表达和减弱总反射 - 里埃变换红外光谱 (ATR-FTIR) 检测甲状腺癌的可行性.
- 评估这些技术在区分各种甲状腺病变的诊断性能.
主要方法:
- 使用从甲状腺组织样本中提取的RNA进行定量实时PCR (qRT-PCR) 的基因表达分析.
- 在甲状腺组织样本上的减弱总反射 - 里埃变换红外光谱 (ATR-FTIR).
- 对ATR-FTIR光谱数据应用的多变量分析.
- 分析包括非新发性 (NN),乳头甲状腺癌 (PTC),卵泡甲状腺癌 (FTC) 和良性瘤 (BT) 的样本.
主要成果:
- 在恶性瘤样本中观察到明显较低的FOS和JUN基因表达,与非新生和良性样本相比.
- 与多变量分析相结合的ATR-FTIR光谱学在识别难以诊断的卵泡性甲状腺癌 (FTC) 中取得了93%的效率.
- 结合分子和光谱方法,在不同的甲状腺病理中显示出诊断潜力.
结论:
- FOS和JUN基因表达水平可以作为甲状腺癌诊断中的潜在生物标志物.
- ATR-FTIR光谱是准确区分甲状腺瘤的宝贵工具,特别是FTC.
- 分子生物学技术和ATR-FTIR光谱的整合对改善差异化甲状腺癌和良性瘤的诊断具有显著的前景.
相关概念视频
Synthesis and Regulation of Thyroid Hormones
4.7K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.7K
IR Frequency Region: Fingerprint Region
937
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
937
The Thyroid Gland
4.0K
The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...
4.0K
Atomic Fluorescence Spectroscopy
419
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
419
Total Internal Reflection Fluorescence Microscopy
5.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.8K
Functions of Thyroid Hormones
2.8K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.8K


