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通过ASIC1a-CMPK2-介导的M1巨细胞两极分化通过IL-18加剧了骨关节炎中的冠状细胞衰老

Lei Dong¹, Yingjie Zhao², Cheng Sun¹

¹Department of Clinical Pharmacology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei 230032, China.

International immunopharmacology

|September 3, 2023

概括

酸感应离子通道1a (ASIC1a) 驱动M1巨细胞极化和IL-18的产生,导致骨关节炎 (OA) 冠状腺细胞衰老. 向ASIC1a可以通过减少M1巨细胞和IL-18.8来治疗OA.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
分子医学是分子医学.

背景情况:

骨关节炎 (OA) 的特点是软骨退化和软骨细胞衰老.
通过炎症信号传递,M1巨细胞有助于OA的病变发生.
酸感应离子通道在OA相关炎症中的作用尚不清楚.

研究的目的:

调查酸感应离子通道1a (ASIC1a) 在M1巨分化中的作用.
阐明ASIC1a影响OA中的冠状细胞衰老的机制.
评估ASIC1a作为OA的潜在治疗点.

主要方法:

在OA患者的突M1巨细胞中进行ASIC1a表达分析.
使用小鼠OA模型评估ASIC1a在M1巨细胞和冠状细胞衰老中的作用.
用RNA测序来分析巨细胞中的基因表达变化.

主要成果:

在OA突M1巨中,ASIC1a的表达上调.
细胞外酸化通过ASIC1a-CMPK2轴促进M1极化,增加IL-18.
IL-18和酸激活的巨细胞诱导胆细胞衰老;ASIC1a的抑制减少了小鼠的OA进展.

结论:

通过ASIC1a介导的M1巨细胞两极分化和IL-18生产驱动OA状细胞衰老.
ASIC1a-CMPK2轴是M1巨分化的一个关键调节器.
ASIC1a代表了OA和其他M1巨细胞介导疾病的有前途的治疗标.

关键词:

在ASIC1a的基础上,ASIC1a是ASIC1a.冠状细胞 (chondrocytes) 是一种细胞.在IL-18中,IL-18是最重要的.在M1巨细胞中.骨关节炎是一种骨关节炎.衰老是一种衰老.

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