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通过过度表达AcGGP3增加了酸合成,改善了基维果中的铜毒性

Xiaoying Liu¹, Chang Cheng², Yan Min³

¹CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.

Journal of hazardous materials

|September 3, 2023

概括

铜 (Cu) 肥料的使用可能会导致果的污染. 在果中增加酸 (AsA) 有效地降低了Cu的毒性,并保护了植物健康.

科学领域:

植物科学植物科学
农业科学农业科学
生物化学生物化学

背景情况:

铜 (Cu) 肥料和农药可以在果中积累,红色和黄色品种由于易受疾病而呈现较高水平.
铜的毒性对果的生产和质量构成重大风险.

研究的目的:

调查 Askorbic 酸 (AsA) 在缓解果中铜毒性的作用.
为了探索基因机制的基础上AsA生物合成,以应对铜压力.

主要方法:

在果上进行了铜应力测试和转录分析.
确定了参与AsA生物合成的关键基因,即GDP-L-银酸酶3 (GGP3) 和GDP-曼诺-3',5'-表皮酶 (GME).
在转基因果中,GGP3过度表达,以评估其对AsA水平和铜耐受性的影响.

主要成果:

铜处理显著增加了AsA含量,并在叶中调节了AsA生物合成基因 (GGP3,GME).
转基因基因果中GGP3的过度表达导致了更高的内源性AsA水平.
增加的AsA通过减少活性氧物种,甲基和电解质泄漏,有效地减轻了铜的毒性,同时保留了叶绿体结构和光系统II的功能.

结论:

亚酸 (AsA) 在保护基维果免受铜毒性的过程中起着至关重要的作用.
通过像GGP3过度表达这样的策略来提高内源的AsA水平,为改善植物中铜应激提供了一种新的方法.

关键词:

在 AcGGP3 基因.亚斯科布酸是一种亚斯科布酸.铜的毒性铜的毒性果 (Kiwi) 是一种果.有反应性氧物种的反应性氧物种.

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