戈尔吉α-曼诺基达酶调节细胞表面的N-甘氨酸类型和跨膜蛋白酶科林的ectodomain脱落
Hao Wang1, Yi-Shi Liu2, Yingfei Peng1
1Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
The Journal of biological chemistry
|September 3, 2023
概括
戈尔吉α-mannosidases对于通过防止ectodomain脱落来维持角质细胞表面表达和活性至关重要. 抑制这些酶会损害冠状腺功能,使心肌细胞对损伤敏感.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 科林是一种跨膜蛋白酶,对于激活尿性来说至关重要.
- 生体主区的脱落和细胞表面表达的减少破坏了角质的功能.
- N-甘氨酸在角质外域脱落中起作用,但Golgi α-曼诺酶的参与尚不清楚.
研究的目的:
- 研究戈尔吉α-曼诺基酶 (I和II) 在角质生物合成,表达和功能中的作用.
- 为了确定抑制戈尔基α-曼诺基达酶如何影响角质外皮膜脱落和细胞表面稳定性.
- 评估改变的冠状腺功能对心肌细胞活力的影响.
主要方法:
- 使用HEK293和AC16细胞系稳定地表达.
- 给药的基富伦辛和斯瓦因素分别抑制了戈尔吉α-曼诺酶I和II.
- 在细胞溶解物和条件介质上进行了西方斑分析.
- 进行了细胞活力测定,包括对过氧化引起的损伤的评估.
主要成果:
- 戈尔基α-曼诺酶I和II都对细胞表面的复杂N-甘氨酸形成有必要.
- 这些酶的抑制会增加在特定领域的冠状体外主区脱落.
- 在α-曼诺酶抑制后,心肌细胞活力下降,这表明因介导的对损伤的敏感性.
- 抑制ectodomain脱落部分恢复了冠状腺细胞因子激活.
结论:
- 戈尔吉α-曼诺基达酶是角质细胞膜恒温的关键调节者.
- 这些酶对N-甘氨酸进行适当的处理,可以保护角氨酸免于脱落,并保持其酶活性.
- 向戈尔吉α-曼诺酶会影响角膜功能和细胞对压力的反应,突出显示它们的治疗潜力.
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