亲和选择质谱测量加速药物发现
Renaud Prudent1, Hugues Lemoine1, Jarrod Walsh2
1Edelris, Bioparc, Bioserra 1 Building, Lyon, France.
Drug discovery today
|September 3, 2023
概括
亲和选择质谱 (AS-MS) 正在成为药物发现和成功识别的强大工具. 它与DNA编码图书馆的协同作用为识别具有挑战性的标的小分子结合剂提供了新的途径,包括RNA.
科学领域:
- 生物化学和药物化学 医学化学
- 分析化学和质谱学.
- 药物发现和开发 药物发现和开发
背景情况:
- 亲和选择质谱 (AS-MS) 在现代药物发现管道中变得越来越重要.
- 该技术提供了一种强大的方法来识别与特定生物标结合的小分子.
- 它与其他尖端技术的整合潜力,如DNA编码图书馆,是一个关键的兴趣领域.
研究的目的:
- 审查AS-MS的兴起,作为打击识别策略中的范式转变.
- 探索AS-MS和DNA编码图书馆技术之间的协同潜力.
- 讨论AS-MS应用于具有挑战性的药物点和RNA点等新型研究领域的最新进展.
主要方法:
- 审查最近的文献和展示AS-MS应用的案例研究.
- 分析AS-MS与DNA编码图书馆的协同作用,以识别成功.
- 基于合同研究组织 (CRO) 和大型制药公司的经验,讨论战略实施.
主要成果:
- AS-MS在识别对困难目标的成功中取得了显著的成功.
- 将AS-MS与DNA编码库集成,提高了打击发现的效率和范围.
- 该技术的多功能性通过其在各种研究领域的应用得到了突出展示,包括RNA-小分子相互作用.
结论:
- AS-MS是一种通用而强大的技术,用于药物发现中的成功识别.
- 它与DNA编码库相结合,为应对具有挑战性的目标提供了一个有希望的策略.
- 未来的研究方向包括将AS-MS应用扩展到RNA向等新领域.
更多相关视频
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.7K
07:33Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
14.3K
相关概念视频
Affinity Chromatography
731
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
731
Peptide Identification Using Tandem Mass Spectrometry
6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K
MALDI-TOF Mass Spectrometry
4.9K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.9K
Mass Spectrometry: Complex Analysis
820
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
820
Mass Spectrometers
5.6K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.6K
Drug Discovery: Overview
8.0K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
8.0K
