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Updated: Aug 19, 2026

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一种无酶的方法,用于从动物模型中分离丸巨细胞

Wei Cai1, Yalong Yang2

  • 1Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Journal of immunological methods
|September 3, 2023
PubMed
概括

这项研究引入了一种新的,无酶的方法来隔离丸巨细胞 (TMs),这是丸中的关键免疫细胞. 这种技术保留了他们的免疫特征,提供了一种更准确的方法来研究TM在生殖健康中的功能.

关键词:
酶消化过程中的消化酶.免疫简介 免疫简介 免疫简介丸的巨细胞

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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...