使用DAP-seq-ML对五种与树木木材发育相关的R2R3-MYB转录因子进行功能性研究
Lazarus T Takawira1, Ines Hadj Bachir2, Raphael Ployet1
1Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa.
Plant molecular biology
|September 3, 2023
概括
这项研究确定了关键的R2R3-MYB转录因子,调节了树中木材的形成. DAP-seq-ML方法准确地确定了目标,揭示了EgrMYB137在植物中促进了二次细胞壁化.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 林业林业 林业 林业 林业
背景情况:
- 转录网络控制植物二次细胞壁 (SCW) 的形成,在物种之间存在差异.
- R2R3-MYB转录因子 (TFs) 是SCW发育的关键调节者.
研究的目的:
- 调查五种树R2R3-MYB TFs在木材形成中的作用.
- 使用DAP-seq-ML.识别全基因组TF结合部位和直接向基因.
主要方法:
- 在5个Eucalyptus R2R3-MYB TF中应用了改进的DAP-seq协议.
- 开发了一种机器学习分类器 (DAP-seq-ML),用于可靠的目标基因分配.
- 通过转基因植物的过度表达和抑制实验验验证了这些发现.
主要成果:
- 与基线方法相比,DAP-seq-ML增强了目标基因预测准确性和回忆力.
- 对于EgrMYB1,EgrMYB2和EgrMYB137的预测目标,对SCW相关的过程进行了丰富.
- EgrMYB137过度表达增加了木质化;抑制减少了它并阻碍了生长.
结论:
- DAP-seq-ML有效地识别了Eucalyptus中生物相关的R2R3-MYB目标.
- EgrMYB137在植物中促进SCW木质化方面发挥着重要作用.
- 在EgrMYB137和EgrMYB2.2之间存在部分功能冗余.
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