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在Halomonas sp.中优化聚酸酸的生产. YLGW01使用混合挥发性脂肪酸:关于混合物分析和食批量策略的研究

Yerin Park^1,2, Jong-Min Jeon¹, Jea-Kyung Park¹

¹Green & Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan-si, Chungnam, 31056, Republic of Korea.

Microbial cell factories

|September 3, 2023

概括

使用Halomonas sp.优化了多基酸盐 (PHA) 的生产. 含有挥发性脂肪酸的 YLGW01. 一个pH-stat养批量策略显著提高了PHA产量,达到5.1g/L.

科学领域:

生物技术和微生物工程
聚合物科学与工程聚合物科学与工程
可持续发展的材料开发.

背景情况:

聚酸酸 (PHAs) 是可生物降解的聚合物,有可能取代石油基塑料.
可再生生物质来源为PHA生产提供了一个可持续的途径.
优化微生物菌株和发酵条件对于高效的PHA合成至关重要.

研究的目的:

为了优化Halomonas sp.产生的多基酸盐 (PHA) 生产. YLGW01使用混合挥发性脂肪酸 (VFA).
研究碳和源优化对PHA产量的影响.
评估不同的料批策略,以提高PHA生产力.

主要方法:

优化VFA比率 (乙酸与乳酸) 和对有机源的选.
应用溶解氧 (DO) - 统计,VFA消费率统计和pH - 统计养批量培养方法.
在各种发酵条件下分析细胞干量 (CDW) 和PHA含量.

主要成果:

最佳的VFA比率 (乙酸:乳酸0.45:0.55) 产生了3.15g/L的CDW和1.63g/L的PHA.
有机源 (豆) 补充剂增加了CDW至2.32g/L和PHA至1.60g/L.
在68小时内,pH-stat养批量策略实现了7 g/L CDW和5.1 g/L PHA (73%的PHA含量).

结论:

混合物分析和有机源选对于优化PHA生产是有效的.
在pH-stat养批量策略显著提高PHA生产力在Halomonas sp. YLGW01使用VFA.
这项研究证明了一种可行的微生物发酵途径,用于可持续的PHA生产.

关键词:

哈洛蒙纳斯 sp. 的一种.聚氨酸多基甲基酸挥发性脂肪酸是一种挥发性脂肪酸.

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