活细胞内源性RNA的成像用一种基因编码的化类菌性阿帕特梅尔
Yan Peng1,2, Linjuan Shu3, Xiongfei Deng1,2
1Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, P. R. China.
Analytical chemistry
|September 4, 2023
概括
研究人员开发了一种新的遗传编码传感器,用于在活细胞中进行敏感RNA成像. 这种原性全osteric aptamer (FaApt) 工具增强了对RNA动态和疾病的理解,有助于诊断和治疗评估.
科学领域:
- 分子生物学分子生物学
- 细胞成像 细胞成像
- 生物技术是生物技术.
背景情况:
- 精确的成像和细胞RNA的跟踪对于理解RNA功能和开发疾病疗法至关重要.
- 现有的工具往往缺乏足够的生物相容性,特异性或敏感性,以适用于活细胞应用.
研究的目的:
- 开发一种新的基因编码传感器,用于敏感的成像和追踪活细胞内源性RNA.
- 为了证明传感器在监测mRNA表达变化和确认RNA在生物系统中的局部化方面的实用性.
主要方法:
- 开发一种原性全性阿帕特默 (FaApt) 传感器系统.
- 利用互补的双重组形成来诱导用于目标RNA识别的aptamer构造变化.
- 在人类癌细胞和斑马鱼胚胎中应用FaApt传感器用于RNA成像和跟踪.
主要成果:
- 该FaApt传感器能够对活细胞中的RNA定位和动态进行敏感的成像.
- 成功监测了瘤亡因子和小分子抑制剂对CXCL1和幸存者mRNA水平的影响.
- 证实了内源性Squint mRNA在发育中的斑马鱼胚胎中的不对称分布.
结论:
- 开发的FaApt传感器是一种有效的分子工具,用于敏感的成像和追踪活细胞内源性RNA.
- 传感器的高特异性和小尺寸为早期诊断与RNA标记物相关的疾病提供了潜在的应用.
- 预计它将促进治疗治疗过程的实时评估.
相关概念视频
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
02:11Reporter Genes
11.5K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.5K