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Updated: Jul 14, 2026

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使用免疫光显微镜在体马的突和腹膜液样本中可视化中性粒细胞外陷

Emily M Birckhead¹, Shubhagata Das¹, Naomie Tidd²

¹School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

|September 4, 2023

概括

中性细胞外细胞陷 (NETs) 可以帮助诊断马匹的败血性炎症. 这项研究可视化了马的突和腹腔液中的NET,有助于检测感染过程.

科学领域:

兽医医学兽医医学兽医医学
免疫学免疫学免疫学
马的健康马的健康

背景情况:

马的败血性鼻膜炎和腹膜炎是通过临床发现和关节和腹部液体的实验室分析来诊断的.
由于实验室结果重叠,区分败血性和非败血性炎症可能具有挑战性.
中性细胞外细胞陷 (NETs) 是天生的免疫反应的一个组成部分,但它们在马的败血症状况中的作用尚未被探索.

研究的目的:

为了确定是否可以在败血症马的阴膜液和腹膜液样本中可视化NET.
评估免疫光向素化组合素H3 (Cit-H3) 和骨髓氧化酶 (MPO) 对于NET检测的有用性.

主要方法:

来自马的9个突液和4个腹液样本的分析.
使用抗体对抗Cit-H3和MPO的免疫光染色.
使用细胞胺涂抹的计数技术量化NET百分比.

主要成果:

在所有分析的污水样本中都明显出现了NET.
在非败菌对照样本中没有NET.
与MPO相比,使用Cit-H3免疫标记更清楚地可视化了NET.

结论:

NETs显示出潜在的生物标志物用于诊断马匹的败血性炎症.
免疫光,特别是Cit-H3是一种可行的方法,用于在马类临床样本中可视化NET.
对NET和相关标记物的进一步调查可以改善对马类败血症的理解和诊断.

关键词:

网络网络网络网络基因组基因组基因组马匹马匹马匹免疫光效应免疫光效应感染的感染感染.腹腔周围的液体突液是指突液中的液体.

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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.