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在芽酵母中,Cdc42对 septin 通过 Axl2 通过轴向地标组合的招募
bioRxiv : the preprint server for biology
|September 4, 2023
概括
发芽的酵母细胞使用地标来指导极化生长. 这项研究揭示了Cdc42 GTPase和Axl2蛋白如何将这一里程碑与轴向芽的隔膜招募联系起来.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞极化对于细胞分裂和分化至关重要,通常由空间线索指导.
- 在芽酵母 (Saccharomyces cerevisiae) 中,单体细胞表现出轴向芽,这个过程涉及特定的地标,如Bud3,Bud4,Axl1和Axl2.
- 七素,状形成的GTPases,对于芽是必不可少的,但它们对轴向标志的精确招募机制尚未完全理解.
研究的目的:
- 阐明在Saccharomyces cerevisiae中芽过程中septins被招募到轴向地标的机制.
- 调查Cdc42 GTPase和Axl2在这个招聘过程中的作用.
主要方法:
- 同免疫沉测试检测蛋白质与蛋白质相互作用.
- 对Cdc42和septin组件有缺陷的酵母突变的分析.
- 显微镜观察隔膜局部化和细胞形态.
主要成果:
- Axl2与Bud3和Cdc42.4的GTP结合形式相互作用.
- Axl2还与隔膜子单元Cdc10相互作用,促进隔膜的招募.
- 一种cdc42突变显示了Axl2相互作用的减少和隔膜招募的受损,特别是在G1阶段.
结论:
- 提出一个模型,其中活跃的Cdc42 GTPase将Axl2招募到Bud3-Bud4复合体中.
- Axl2 作为一个链接器,与 Cdc10 相互作用,将轴向地标连接到隔膜招募.
- 这种机制确保了隔膜的适当空间组织,以便在酵母中进行轴向芽.
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