两个功能上不同的HEATR5蛋白质复合体是由酵母中的快速进化的辅助因子定义的
Lucas J Marmorale1,2, Huan Jin1, Thomas G Reidy1,3
1Department of Cell and Developmental Biology, University of Michigan, Ann Arbor MI.
bioRxiv : the preprint server for biology
|September 4, 2023
概括
发芽酵母Laaa1蛋白在两个不同的复合体中起作用,揭示了HEATR5蛋白如何结合辅助因子. 这些发现揭示了Laa1的存在.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- HEATR5蛋白对于膜流通至关重要,它与适应蛋白复合体-1 (AP1) 相互作用.
- 通过HEATR5蛋白质参与它们快速演变的辅因子的精确机制在很大程度上仍未被阐明.
研究的目的:
- 阐明HEATR5蛋白与其辅助因子相互作用的机制.
- 研究发芽酵母HEATR5蛋白,Laa1及其结合伙伴的独特功能.
主要方法:
- 蛋白质复合体的生物化学分析.
- 蛋白质相互作用的结构预测.
- 在芽酵母 (Saccharomyces cerevisiae) 的功能研究.
主要成果:
- Laa1在两个不同的综合体中运作,具有相互排斥的有约束力的合作伙伴 Laa2和 Lft1.1.
- 尽管序列同样性有限,但Lft1和Laa2通过结构相似的机制结合Laa1.
- 这两个Laa1复合体都参与了Golgi内部的回收.
- 只有Laa2-Laa1复合体直接结合并影响AP1.1的局部化.
- 结构预测表明,人类HEATR5蛋白及其辅助因子的结合机制得到保护.
结论:
- 阐明了在酵母和人类中HEATR5辅因子结合的保存,结构相似的机制.
- 证明Laa1具有超出AP1招募的功能,扩大了我们对其细胞作用的理解.
- 提供了对HEATR5蛋白相互作用及其对膜贩运的影响的机制性见解.
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