相关实验视频
Updated: Jul 17, 2025

07:39
Mouse In Vivo Placental Targeted CRISPR Manipulation
Published on: April 14, 2023
2.7K
在室内小鼠中对胎盘基因表达产生原始母体影响的演变
bioRxiv : the preprint server for biology
|September 4, 2023
概括
基因组印记对于胎盘发育至关重要,在小鼠中迅速发展. 组织专业化影响印记基因表达,推动物种之间的进化分歧.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 发育生物学是发展生物学.
背景情况:
- 基因组印记,即特定于父母的基因沉默,对于哺乳动物的胎盘发育和物种间的繁殖障碍至关重要.
- 印记进化的速度和胎盘组织专业化的作用仍然不太清楚.
研究的目的:
- 调查基因组印记在密切相关的小鼠血统中跨不同胎盘组织的进化.
- 确定胎盘层的功能专业化如何影响印制的基因表达模式.
主要方法:
- 在功能上不同的胎盘层中,对三个Mus血统中相互交叉的胎盘层表达偏差的比较分析.
- 基因表达全基因组和胎儿和母体组织的DNA甲基化概况.
- 开发一种分析策略,以减轻胎盘样本中的母亲污染偏差.
主要成果:
- 验证了42个已知的印记基因的印记,并确定了5个新型候选人.
- 在迷宫区 (营养物转移) 中丰富的父性偏见表达;在交叉区 (母体生理调节) 中的母性偏见表达.
- 不同甲基化区域主要涉及母体表观遗传修饰,与母体和父体偏差相关;在M. m. domesticus.中观察到印记状态的快速分歧.
结论:
- 胎盘组织的专业化与印记基因表达的进化有关.
- 在密切相关的动物物种中,印记发生了快速的进化变化.
- 研究结果提供了对胎盘生物学与基因组印记进化的相互作用的见解.
相关概念视频
Genomic Imprinting and Inheritance
34.6K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
34.6K
Background and Environment Affect Phenotype
6.6K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.6K
Position-effect Variegation
6.4K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.4K

