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协酶B12前体5,6-二甲基米达是沙门氏菌中的一种flavin对手

Lahiru Malalasekara¹, Jorge C Escalante-Semerena¹

¹Department of Microbiology, University of Georgia, Athens USA.

Microbial cell (Graz, Austria)

|September 4, 2023

概括

5,6-二甲基胺醇 (DMB) 通过模仿黄素辅因子来抑制沙门氏菌 Typhimurium 的生长. 基质载体中的突变减轻了这种抑制,这表明DMB干扰营养吸收.

科学领域:

微生物学微生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

沙门氏菌 Typhimurium 在无氧条件下合成腺甲胺 (AdoCbl),但可以在空气中组装其核酸环和C-Co键.
在核酸环组装研究中,观察到较低的配体核基,5,6-二甲基胺醇 (DMB),可以阻止S. Typhimurium的生长.

研究的目的:

研究5,6-二甲基胺醇 (DMB) 抑制沙门氏菌Typhimurium生长的机制.
确定DMB与黄辅因子的结构相似性是否是其对黄酶的抑制作用的基础.

主要方法:

在体外和体内研究的DMB抑制在家政flavin脱酶 (Fre) 和代谢性flavoenzymes.
使用各种基质 (三甲基酸盐,酸盐,D-氨酸,糖醇) 和胺衍生物的生长测定.
自发的DMB耐药突变菌株的全基因组测序.

主要成果:

DMB通过结构模仿黄辅因子来抑制生长,从而影响黄酶的活性.
通过三碳酸盐,酸盐或D-alanine,DMB抑制了生长,而5-甲基胺醇则具有差异效应.
在cycA,dctA和TcuC载体中的突变赋予了耐药性,这表明基质吸收影响DMB抑制.

结论:

由于DMB与黄素的结构相似,导致S. Typhimurium的生长受阻.
增加基质运输可以克服DMB介导的生长停止.
提出了两个潜在的DMB抑制机制,涉及黄酶干扰和基质吸收.

关键词:

5,6-二甲基二米达.CoB12生物合成 CoB12生物合成班齐米达抗微生物药物胺基核基是胺基核基.酵素抑制剂酵素抑制剂

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