相关实验视频

Updated: Jul 17, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

在肝癌中差异表达的非编码RNA及其调节网络

Nurbubu T Moldogazieva¹, Sergey P Zavadskiy¹, Dmitry V Astakhov²

¹Department of Pharmacology, Nelyubin Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, 119991, 8 Trubetskaya str., Moscow, Russia.

|September 4, 2023

概括

非编码RNAs (ncRNAs),包括microRNAs (miRNAs),长非编码RNAs (lncRNAs) 和圆形RNAs (circRNAs),在肝癌中至关重要. 差异表达的ncRNA及其网络在肝癌发生过程中发挥作用,并可作为诊断生物标志物.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
癌症研究癌症研究

背景情况:

非编码RNAs (ncRNAs),如microRNAs (miRNAs),长非编码RNAs (lncRNAs) 和圆形RNAs (circRNAs),是基因表达的关键调节者.
这些ncRNAs形成复杂的调节网络,包括竞争性内源RNA (ceRNA) 网络,影响细胞过程.
对ncRNAs的异常表达越来越多地与各种癌症有关,包括肝癌.

研究的目的:

审查最近在理解差异表达的ncRNAs (DEMs,DELs,DECs) 及其网络在肝癌中的作用方面的进展.
探索这些ncRNAs在关键癌症特征中的参与,如细胞信号传递,细胞循环和细胞亡.
讨论ncRNAs作为肝癌诊断和预后的生物标志物的潜力.

主要方法:

对最近关于肝癌发生的ncRNA研究的文献综述.
对ncRNA调节机制的分析,包括miRNA-mRNA相互作用和ceRNA网络.
检查差异表达的ncRNAs在肝癌发育和进展中的功能作用.

主要成果:

在肝癌中,ncRNAs显著影响细胞信号传递,细胞循环,转录,血管生成,细胞亡和瘤微环境重塑.
不同表达的miRNAs,lncRNAs和circRNAs可以作为瘤基因或瘤抑制剂.
在肝癌发生过程中,ncRNA网络参与了代谢重编程.

结论:

ncRNAs是肝癌发病过程中的关键参与者,影响多个细胞通路.
ncRNAs作为早期诊断和肝癌预后的潜在生物标志物具有前途.
需要进行进一步的研究,以克服在肝癌临床应用中有效利用ncRNAs方面的挑战.

关键词:

肝细胞癌是肝细胞癌.在肝脏内发生的胆管癌,胆管癌.监管网络监管网络是指监管网络.环RNA 环RNA 是一个环RNA.在ncRNA中,我们可以这是一个小RNARNA.

更多相关视频

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers

Author Spotlight: Impact of Intergenic Interactions on Disease-Identifying Dark Biomarkers

Published on: March 1, 2024

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...