猪面部皮肤发育的全面转录分析
Yujing Li1, Rui Shi1, Rong Yuan2
1Department of Zoology, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan, China.
PeerJ
|September 4, 2023
概括
这项研究揭示了猪皮肤发育过程中的关键RNA变化,识别了调节皮肤结构和功能的PPAR信号传递等基因和途径. 这些发现提升了我们对皮肤发育机制的理解.
科学领域:
- 基因组学和分子生物学
- 发展生物学 发展生物学
- 比较解剖学的比较.
背景情况:
- 猪皮肤是人类皮肤外移植的有价值模型,因为它们的解剖学和生理学相似性.
- 在所有猪品种中存在不同的皮肤发育模式,一些中国品种表现出独特的厚度和面部折叠特征.
- 控制猪皮肤发育的精确监管机制在很大程度上仍未得到阐明.
研究的目的:
- 调查猪皮肤发育过程中的动态RNA表达变化.
- 在不同发育阶段识别差异表达的信使RNAs (mRNAs),长非编码RNAs (lncRNAs),microRNAs (miRNAs) 和圆形RNAs (circRNAs).
- 阐明这些RNA和相关基因在皮肤发育中的功能作用.
主要方法:
- 在四个发育阶段的成华母猪的面部皮肤样本上利用RNA测序 (RNA-seq):产后第3天 (D3),第90天 (D90),第180天 (D180) 和第3年 (Y3).
- 对mRNAs,lncRNAs,miRNAs和circRNAs进行了差异表达分析.
- 进行了基因本体学 (GO) 丰富和基因和基因组的京都百科全书 (KEGG) 路径分析以进行功能注释.
- 构建的 lncRNA-miRNA-mRNA 和 circRNA-miRNA 相互作用网络.
主要成果:
- 确定了许多差异表达RNA (DERNA),它们的数量随着发育时间的推移而增加.
- 观察到持续下调拉斯 (ELN) 表达,影响皮肤弹性的关键成分.
- 丰富的途径包括PPAR信号通路,这对于皮肤发育至关重要,跨越多个发育比较.
- lncRNA-miRNA-mRNA和circRNA-miRNA相互作用网络揭示了潜在的调节关系.
结论:
- 确定了涉及猪皮肤发育的新基因和调节网络.
- 功能分析提供了对差异表达基因作用的见解.
- 构建的交互网络作为未来研究皮肤发育机制的基础.
相关概念视频
Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...


