关于RUNX2沉默对SJSA-1骨髓瘤细胞中转录和代谢学特征的影响的数据集
Mai Nhu Uyen Le1, Ruiqi Chen2, Liang-E Xia3
1State Key Laboratory of Developmental Biology of Freshwater Fish & Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Science, Hunan Normal University, Changsha, Hunan 410081, China.
Data in brief
|September 4, 2023
概括
在骨髓癌细胞中,RUNX2基因沉默改变了基因表达和代谢. 这项研究提供了了解RUNX2的数据.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 代谢学 代谢学 代谢学
背景情况:
- 骨髓瘤是一种普遍存在的原发性骨癌,具有显著的转移和复发潜力.
- 代谢重编程是癌症的关键标志,包括骨髓瘤,通常是由遗传和表观遗传变化驱动的.
- 骨质细胞分化中的关键转录因子RUNX2在异常表达时,与骨质肉瘤的发展和进展有关.
研究的目的:
- 调查RUNX2基因沉默对骨髓瘤细胞的转录和代谢概况的影响.
- 在RUNX2敲击后生成和分析基因表达和代谢物变化的全面数据集.
- 为了促进新型RUNX2标的识别,并阐明其在骨髓瘤病原和代谢重编程中的作用.
主要方法:
- 产生具有稳定的RUNX2shRNA表达和控制混杂shRNA表达的SJSA-1骨髓瘤细胞系.
- 使用Illumina NovaSeq 6000进行转录组分析,以分析基因表达变化.
- 使用超高性能液态染色学结合飞行时间质谱法 (UHPLC-TOF-MS) 分析代谢物变化进行代谢组概况.
主要成果:
- 在骨髓癌细胞中抑制RUNX2导致了转录组和代谢组概况的显著变化.
- 对比分析揭示了RUNX2-沉默和对照细胞之间的明显的基因表达模式和代谢特征.
- 生成的数据集为识别由RUNX2.2调节的基因和代谢途径提供了宝贵的资源.
结论:
- 在骨髓瘤中,RUNX2在调节基因表达和代谢重编程方面发挥着至关重要的作用.
- 通过转录组和代谢组分析了解RUNX2的功能,可以揭示新的治疗点.
- 该研究为进一步研究骨髓瘤发展和进展背后的机制提供了基础数据.
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